FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172584
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT155339.000254948315023No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT152188.81773513187781No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT144938.397649840077875No Hit
ATGGACCGTGTCTCAGTTCCATTGTGGGGGACCTTCCTCTCAGAACCCCT110236.387034719325083No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT84664.905437352245863No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTGAGCCTCTCAGCTCGGCT78984.57632225467019No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACTCTCTCAAGTCGGCT69164.007323969777036No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT60613.5119130394474576No Hit
ATGGACCGTGTCTCAGTTCCATTGTGGGGGATCTTCCTCTCAGAACCCCT45652.645088768367867No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT43702.532100310573402No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCGTCCTCTCAGACCAGCT39412.2835257034255783No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT35832.076090483474714No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT33941.9665785936123856No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT30961.7939090529828954No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT23161.3419552218050341No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT21661.2550410235015992No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT21161.226069624067121No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT18611.0783154869512817No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT16430.9520001854169563No Hit
ATGGGCCGTGTCTCAGTCCCATTGTGGGGGGCCTTCCTCTCAGAACCCCT16240.9409910536318545No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT15390.8917396745932415No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT15300.8865248226950355No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT14020.812358040142771No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT12850.7445649654660919No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT9360.5423445974134334No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT8690.5035229221712326No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCAGCCTCTCAGCTCGGCT8080.4681778148611691No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT7290.42240300375469336No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT6900.39980531219580034No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTTCGGCT6670.3864784684559403No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT6450.37373105270476986No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT6350.3679367728178742No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT6320.3661984888518055No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT6130.35518935706670374No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT6120.3546099290780142No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACTCTCTCAAGTCGGCT6000.3476567932137394No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT5830.33780651740601675No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATTAACCTCTCAGTTCGGCT5660.3279562415982941No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTTCGGCT5620.3256385296435359No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT5420.3140499698697446No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTCCGGCT5200.30130255411857415No Hit
ATGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT5100.29550827423167847No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT5030.2914522783108515No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAAGTCGGCT4880.282760858480508No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT4750.27522829462754367No Hit
TTGGACCGTGTCTCAGTTCCATTGTGGGGGACCTTCCTCTCAGAACCCCT4400.2549483150234089No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACGCTCTCAAGTCGGCT4000.23177119547582625No Hit
CTGGACCGTCTCTCAGTTCCAGTGTGGCCGGTCGACCTCTCAGTCCGGCT3880.2248180596115515No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT3870.22423863162286192No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT3620.20975293190562275No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT3590.20801464793955407No Hit
CTGGACCGTGTTTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT3230.18715524034672973No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCTACCTCTCAGTACGGCT3050.17672553655031753No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTACATCCTCTCAGATCGGCT2760.1599221248783201No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT2650.1535484170027349No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTGAGCCTCTCAGCTCGGCT2620.15181013303666618No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT2580.14949242108190794No Hit
CTGGACCGTGTCTCAGTTCCATTGTGGGGGACCTTCCTCTCAGAACCCCT2570.14891299309321837No Hit
CTGGACCGTTTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2550.14775413711583923No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT2540.14717470912714967No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT2450.14195985722894358No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTTCGGCT2420.1402215732628749No Hit
CTGGGCCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT2230.12921244147777314No Hit
TTGGACCGTTTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2210.128053585500394No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2080.12052102164742966No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTTAACCTCTCAGTCCGGCT2040.1182033096926714No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCACTCTCTCAAGTCGGCT1960.11356788578315487No Hit
ATGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT1950.11298845779446531No Hit
TTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1930.11182960181708616No Hit
CTGGACCGTCTCTCAGTTCCAATGTGACCGGTCAACCTCTCAGTCCGGCT1880.10893246187363835No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT1860.10777360589625921No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCCCGGCT1800.10429703796412182No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT1790.10371760997543225No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT1790.10371760997543225No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT1770.10255875399805313No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT1760.10197932600936356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCTA305.456968E-12295.0295
CCGTGTC134500.0294.780676
GACCGTG59750.0294.753144
CGTGTCT134450.0294.670877
GGACCGT64450.0294.542273
GTGTCTC134600.0294.34258
TGGACCG64450.0294.313422
CGTATCT34400.0294.142467
ACCGTGT59900.0294.0155
TGTCTCA134750.0294.014869
GCCGTGT68200.0293.486085
GTATCTC34550.0292.865428
GGCCGTG68350.0292.841984
TTGGACC20000.0291.31251
CTGGGCC74600.0291.045561
GGCCGTA33200.0291.00154
CTGGACC27600.0290.190221
TTGGGCC25950.0289.3161
GCCGTAT33700.0287.121675
CCGTATC35250.0287.049626
Produced by FastQC (version 0.11.5)