FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148153
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG1696811.453024913434085No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC95866.470338096427342No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG90416.102475143939036No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG88375.964779653466349No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA73204.940838187549358No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG45693.083973999851505No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG43592.942228642011974No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT40382.725560737885834No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG29722.006034302376597No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAG25421.715793807752796No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC18411.242634303726553No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA17791.2007856742691676No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA16591.1197883269322928No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT15881.0718648964246422No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGATA15321.0340661343341007No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT15001.012466841710934No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGCCC14991.01179186381646No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGA14831.0009922175048767No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG14400.9719681680424965No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT14070.9496938975248561No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA10600.7154765681423934No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATC10180.6871274965744872No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC9940.6709280271071123No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT9900.6682281155292165No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG9830.6635032702678987No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT9790.6608033586900028No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATTACA9110.6149048618657739No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA9080.612879928182352No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG9040.6101800166044562No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGTTA8310.5609066303078574No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATACTG8140.5494320061018002No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT8040.5426822271570606No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT7970.5379573818957429No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA7890.5325575587399513No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG7660.5170330671670503No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG7650.5163580892725763No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGT7550.5096083103278368No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA7540.5089333324333628No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT6450.43536074193570157No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA6350.4286109629909621No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAAGAA6100.4117365156291132No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTTATGG5870.3962120240562122No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA5580.37663766511646746No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGGGGCCC5530.37326277564409766No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTGCC5470.36921290827725395No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC5470.36921290827725395No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAA5450.367862952488306No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG5310.35841326196567064No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC5060.34153881460382174No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGA4490.3030650746188063No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC4240.2861906272569573No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG4210.2841656935735355No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG3920.26459133463379075No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTTTT3800.2564915999001033No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG3770.2544666662166814No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA3720.2510917767443116No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA3700.2497418209553637No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGAAGAT3650.24636693148299393No Hit
GACGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC3480.23489230727693666No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGTGGCCC3390.22881750622667107No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA3380.22814252833219711No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT3200.21599292623166588No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA3030.20451830202560867No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGGATGCG2990.20181839044771283No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2980.20114341255323887No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTTTAAC2960.19979345676429094No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA2910.1964185672919212No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATCTTCTTC2800.18899381045270766No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTAAGACG2780.18764385466375977No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA2700.1822440315079681No Hit
GATGAACGCCGGCGGCGTGCCTAATACATGCAAGTCGAGCGGGGCCCGCA2680.1808940757190202No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG2660.1795441199300723No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGTTAAGGCG2550.17211936309085876No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTTAAA2540.17144438519638483No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG2520.1700944294074369No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAACAGGA2470.16671953993506713No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAG2450.16536958414611921No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTCTCTTC2200.14849513678427032No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT2180.1471451809953224No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT2160.14579522520637447No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGGGGCAGCGGGG2120.14309531362847866No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAT1980.13364562310584327No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATCTT1880.12689584416110372No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA1810.12217099889978603No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGCGG1770.1194710873218902No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAATTTCA1690.11407126416609856No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACACT1670.11272130837715064No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA1630.11002139679925482No Hit
GACGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG1620.10934641890478086No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT1520.1025966399600413No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC1510.10192166206556734No Hit
GATGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT1500.10124668417109339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTGT157.2276634E-6295.0295
CACACTC108.4575993E-4295.0295
CAGACCC108.4575993E-4295.0295
ATAAACG8350.0294.999972
CGCTAGC57300.0294.742587
ACGCTAG57250.0294.742346
TGAACGC92850.0294.364533
CGCTGGC90400.0294.347357
AACGCTG90450.0294.184635
CTGGCGG90100.0294.017769
ACGCTGG90650.0293.535556
TAAACGC8400.0293.244053
GCTAGCG36400.0292.97398
CTAGCTA21150.0292.90789
GAACGCT140650.0291.224674
AACGCTA57950.0291.182045
AAACGCT8650.0289.88444
GATAAAC8500.0289.794131
GCTGGCG91750.0289.69488
ACGAACG47950.0287.61732
Produced by FastQC (version 0.11.5)