FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215269
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA3109714.445647074125862No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG95414.432129103586675No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAA92074.2769743901815875No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA74913.4798322099326886No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT68353.1750972039634133No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT67483.1346826528668785No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG46412.1559072602186102No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG44342.0597485007130616No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG43592.0249083704574278No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG42231.961731600927212No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG41821.9426856630541323No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA41131.9106327432189492No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC39951.8558176049500856No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT31771.4758279176286413No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGACGATTG29831.385708114034069No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT27251.2658580659546892No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA26981.253315619062661No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT26911.2500638735721354No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATTATT25551.1868871040419198No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTT24131.1209231240912534No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG22641.0517073986500611No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA20010.9295346752203058No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT17950.8338404507848319No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG17330.8050392764401748No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGCGGCCC16340.7590503045027384No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAGCGGAGATTGAG15720.7302491301580812No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA15630.7260683145274053No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGGGATTACT15040.6986607453929735No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC14670.6814729478001942No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAGTTAGA14600.6782212023096683No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTG13850.6433810720540347No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT13610.632232230372232No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATTACT12720.5908886091355467No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA11840.5500095229689366No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA11290.5244600941148052No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAACATAA11150.5179566031337536No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG10920.507272296522026No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGGGATTTGTA9880.458960649234214No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG9530.44270192178158485No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTT9320.43294668531000746No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG9120.4236559839085051No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACTTTACA9050.42040423841797936No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT8040.3734861963403927No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA7050.3274972244029563No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT6930.32192280356205494No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCAAGAGA6820.31681291779122867No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGTGG6100.2833663927458203No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT5930.2754692965545434No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGGGTGCGTT5700.26478498994281574No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT5670.2633913847325904No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTCTCTTC5470.2541006833310881No Hit
GATGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGCTATTA5400.2508489378405623No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAATTTACA5150.23923556108868438No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC5140.23877102601860928No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT5040.2341256753178581No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG4860.22576404405650605No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG4730.21972508814552955No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA4730.21972508814552955No Hit
GACGAACGCTGGCGGCGTGCTTCATACATGCAAGTCGAACGAGAAGTTAC4630.21507973744477837No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC4610.21415066730462815No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGTGATTTGCT4490.20857624646372674No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG4230.19649833464177377No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA4200.19510472943154844No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAA4190.1946401943614733No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGAAAGTT4110.1909239138008724No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGTTGAGT4070.18906577352057194No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA4000.18581402803004612No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA3950.18349135267967054No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGG3950.18349135267967054No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA3780.1755942564883936No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT3780.1755942564883936No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG3690.17141344085771756No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGAAATCCG3600.16723262522704152No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAA3580.16630355508689126No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT3490.16212273945621525No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG3320.1542256432649383No Hit
GATGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT3200.14865122242403692No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG3170.14725761721381156No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT3090.14354133665321062No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT3060.1421477314429853No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC3030.14075412623275996No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACTTTATA3020.14028959116268483No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGTAAATG2870.1333215651115581No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA2780.12914074948088206No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGTTTAAT2750.12774714427065673No Hit
GACGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA2730.1268180741305065No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTCT2730.1268180741305065No Hit
AGTGAACGCTGGCGGAACGCTTGACACATGCAAGTCGAACGGTAACGCGG2640.12263725849983044No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA2620.12170818835968021No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC2610.1212436532896051No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA2600.12077911821952998No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGACGAGGAG2550.1184564428691544No Hit
GTTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG2540.11799190779907928No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGAACAGG2500.11613376751877884No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGGAA2480.11520469737862861No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGACGATTG2420.11241748695817791No Hit
GATGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG2380.11055934667787745No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA2350.1091657414676521No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAACGAGAGATGAA2330.10823667132750187No Hit
GATGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGAAGCACCTT2320.10777213625742675No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT2290.10637853104720141No Hit
GACGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG2220.10312678555667561No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG2190.10173318034645026No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAGCAAAG2180.10126864527637515No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG2180.10126864527637515No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATGTTT2180.10126864527637515No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG2170.10080411020630003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAG71100.0295.06
CTGGGCC206.173286E-8294.99997295
TGTACGC108.4602577E-4294.999973
CGCTGGC144100.0294.385867
CTAGCTA55750.0294.20639
TGAACGC128200.0294.19463
ACGCTGG144300.0293.97786
CGCTAGC71300.0293.965677
AAACGCT9400.0293.430884
AACGCTG144700.0293.267125
GCTAGCT56450.0291.603188
ACGAACG78950.0289.5822
CGAACGC79200.0289.04043
ATAAACG9500.0288.789462
AACGCTA72650.0288.706155
GCTAGCG15650.0286.517588
TAAACGC9700.0284.355653
GATAAAC9650.0284.30051
GAACGCT214500.0282.553624
GACGAAC80250.0277.171361
Produced by FastQC (version 0.11.5)