FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215269
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3237615.039787428751932No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACTTTCCTCTCAGAACCCCT2900113.47198156724842No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2323110.791614212915004No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT116865.428556828897798No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT97564.532004143652825No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT73643.42083625603315No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT72833.383208915357065No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT59452.7616609915965604No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT58242.7054522481174716No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT48242.2409171780423565No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGAAGAGCCTCTCAGCCCGGCT40401.8767216831034657No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT33271.5455081781399087No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT30061.3963924206457967No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT26891.249134803431985No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT24321.1297492904226805No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT23371.0856184587655444No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT21661.0061829617826998No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT20950.9732009718073666No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT16710.7762381020955177No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGCCGGCT16330.7585857694326633No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT16250.7548694888720624No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT15690.728855524947856No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT15040.6986607453929735No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT12910.5997147754669738No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT11430.5309635850958568No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT11090.5151693927133029No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT10650.4947298496299978No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGCTGTTCGTCCTCTCAGACCAGCT10070.4677868155656411No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT9690.45013448290278674No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT7870.3655891001491158No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT7330.3405042063650595No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT6940.32238733863213004No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT6180.28708267330642123No Hit
ATGGGCCGTGTCTCAGTCCCATTGTGGCTGATCATCCTCTCAAACCAGCT6140.2852245330261208No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT6130.2847599979560457No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACTCTCTCAAGTCGGCT6010.2791855771151443No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT5580.2592105691019143No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT5270.24480998192958578No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCTGATCGTCCTCTCAGACCAGCT4880.22669311419665628No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT4500.20904078153380187No Hit
TTGGGCCGTGTCTCAGTTCCAATGTGGGGGACTTTCCTCTCAGAACCCCT4150.19278205408117285No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACTTTCCTCTCAGAACCCCT4110.1909239138008724No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT4000.18581402803004612No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACGTTCCTCTCAGAACCCCT3720.1728070460679429No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT3690.17141344085771756No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT3460.1607291342459899No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGTCGGCT3440.15980006410583966No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGCCCTCTCAGGCCGGCT3370.15654831861531385No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3270.15190296791456273No Hit
TTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3240.15050936270433737No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3230.15004482763426225No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT3140.1458640120035862No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT3140.1458640120035862No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT3070.1426122665130604No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT3040.14121866130283506No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT2910.13517970539185856No Hit
TTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT2770.12867621441080693No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT2700.12542446892028114No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGTCCGGCT2390.11102388174795257No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGAAGAGCCTCTCAGCCCGGCT2380.11055934667787745No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2350.1091657414676521No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT2350.1091657414676521No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT2300.10684306611727652No Hit
TTGGACCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT2290.10637853104720141No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT2230.10359132062675072No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGGGGACTTTCCTCTCAGAACCCCT2220.10312678555667561No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT2210.10266225048660049No Hit
CTGGACCGTTTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2170.10080411020630003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGCC83350.0295.01
GTGTCTC195300.0294.471348
GGACCGT79350.0294.442353
CCGTGTC195350.0294.244936
GACCGTG77750.0294.241154
GCCGTAT17600.0294.161935
TGTCTCA195550.0294.094859
GCCGTGT112100.0293.947365
TTGGACC68650.0293.710851
GGCCGTG112250.0293.685974
TGGACCG79650.0293.518522
ACCGTGT77950.0293.486185
CCGTATC18500.0292.60816
GGCCGTA17700.0292.500034
CGTATCT18600.0291.034977
GTATCTC18600.0291.034978
CGTGTCT197800.0290.674967
CTGGACC11350.0284.603521
CTGGTCC3900.0283.653841
TTGGGCC47600.0282.295171
Produced by FastQC (version 0.11.5)