FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005103686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148948
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA1583010.627870129172596No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG147619.910169992212046No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT83695.61873942583989No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT53103.5650025512259313No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC51873.482423396084539No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA50143.3662754786905493No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATGC45373.0460294868007627No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA42682.865429545881784No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT38232.56666756183366No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT35232.3652549883180707No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAAGGTT18651.2521148320219138No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG18461.2393587023659263No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC17731.190348309477133No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCATAG17541.1775921798211457No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT16551.1111260305610011No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG15331.0292182506646617No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGCTCA14400.9667803528748288No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT14060.9439535945430619No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGGGAGAATTG14010.9405967183178022No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA13650.9164272094959314No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGTTTT13080.8781588205279695No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC12860.8633885651368263No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT12290.8251201761688642No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT12140.8150495474930849No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGATTT11950.8022934178370975No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCCA11790.791551413916266No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACTTTATA10540.7076295082847706No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATTT10470.7029298815694067No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGTTT9800.6579477401509252No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCTCA9530.6398206085345222No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAATTT9370.6290786046136907No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCATTTT8970.6022235948116121No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT8450.5673120820689099No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG8070.5417998227569354No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA8020.5384429465316756No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATGT7920.5317291940811558No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGAGG7000.4699626715363751No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTT6830.45854929237049175No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC6540.4390794102639847No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA6530.4384080350189328No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC6220.4175954024223219No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGATA6040.40551064801138653No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG5980.40148239654107476No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCCT5640.3786556382093079No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACTTTACA5540.3719418857587883No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA5290.3551575046324892No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATAT5130.3444155007116578No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGAGATAAAT4900.3289738700754626No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAGGGTT4800.32226011762494294No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGATTT4340.2913768563525526No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGG4320.2900341058624486No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATATTT4280.2873486048822408No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTA4120.2766066009614094No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAACGCCAA4110.27593522571635737No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAGCGGAGATTGAG4020.26989284851088974No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTA4010.2692214732658378No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC3960.2658645970405779No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGTTAGAC3900.26183634557026614No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT3710.2490802159142788No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATACCT3670.24639471493407097No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG3400.22826758331766792No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT3380.22692483282756398No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGGTTT3290.22088245562209627No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA3160.21215457743642077No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTTGAACGAAGACATAC3070.20611220023095309No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC2980.20006982302548543No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTACC2940.19738432204527753No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGATCT2930.19671294680022555No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTT2890.19402744582001774No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCATCCC2740.18395681714423825No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC2460.16515831028278324No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAATCT2420.1624728093025754No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTTAGC2420.1624728093025754No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGACGAGGAG2420.1624728093025754No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC2250.151059430136692No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT2200.1477025539114322No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGACTCGGAG2140.1436743024411204No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA2130.1430029271960684No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCC2130.1430029271960684No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATCCATC2080.1396460509708086No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCC2050.1376319252356527No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGTATTTT2030.1362891747455488No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTACCCT2020.13561779950049682No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGGTTT1990.1336036737653409No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGGTTTGG1980.13293229852028896No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCTCA1940.1302467975400811No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG1920.12890404704997718No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGACATAT1890.12688992131482127No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA1850.12420442033461343No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATTT1840.12353304508956146No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT1830.1228616698445095No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTT1800.1208475441093536No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT1740.11681929263904181No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT1670.11211966592367807No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTT1630.10943416494347019No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAGCCAT1630.10943416494347019No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGTTACAC1570.10540591347315842No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATACT1490.10003491151274271No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATCAG1490.10003491151274271No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)