FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250701
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG210268.386883179564501No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG167326.674085863239476No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT138005.504565199181495No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG113804.539271881643871No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG112374.482231821971193No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG109924.3845058456089125No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG92093.6733000666132165No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCT56332.2468996932600986No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGTTAAAT49541.9760591301989223No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA47701.902664927543169No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT43171.7219715916569938No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTAAAT34141.3617815644931612No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA33641.3418374876845325No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAAGGTT32041.278016441896921No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT29771.1874703331857472No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT26051.0390864017295502No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATGT24040.9589112129588634No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAGC22260.8879102995201456No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT21990.877140498043486No Hit
GACGAACGCTGGCGGCGTGCTTCATACATGCAAGTCGAACGCGAATCGGT21260.8480221459028883No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT20700.8256847798772242No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG20630.8228926091240162No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA20230.8069373476771134No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTTGTAAA18710.7463073541788825No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT18420.7347397896298778No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGATGGA18030.7191834097191475No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC17730.7072169636339704No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG17640.7036270298084172No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGCTCA17090.6816885453189256No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG16110.6425981547740137No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT14860.5927379627524422No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGTTACAC13680.5456699414840787No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGGACGCAATGCTTC13380.5337034953989015No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGGGATTTGTG13380.5337034953989015No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGGACGGAAGA12910.5149560631987906No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGCTAGGA12520.4993996832880603No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCGGGA12350.49261869717312656No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG12050.4806522510879494No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGAGATATTT11800.4706802126836351No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA11510.4591126481346305No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCACTT10950.4367752821089665No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTG10920.43557863750044873No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT10700.42680324370465217No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAATGAAGTT10420.4156345606918201No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT9980.3980837731002269No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGGAG9170.36577436867024865No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC8840.3526112779765537No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTAGAG8800.35101575183186345No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG8680.3462291733977926No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAGGGTT8670.34583029186162006No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAACCA8570.34184147649989427No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT8250.32907726734237197No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTATTT8230.32827950427002683No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT7880.31431865050398683No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATATTT7740.3087343089975708No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGTAGCAGGG7060.28161036453783594No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGTAACAGGG6910.2756271414952473No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGATTT6880.2744304968867296No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA6690.26685174769945075No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT6660.26565510309093304No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGGGCAGCA6620.2640595769462427No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACCTT6610.2636606954100702No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGATTATT6480.25847523543982676No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATC6260.24969984164403014No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA6190.24690767089082213No Hit
GACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG6110.24371661860144153No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG5930.23653675095033525No Hit
GACGAACGCTGGCGGCGTGCTTCATACATGCAAGTCGAACGAGAAGCTTA5650.22536806793750325No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGTTAGAC5600.2233736602566404No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCC5110.20382846498418436No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGAGG5100.20342958344801176No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA4980.19864300501394092No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA4970.19824412347776835No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAAAGTTC4970.19824412347776835No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT4930.19664859733307805No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT4850.19345754504369747No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGCTT4700.1874743220011089No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGGACGGAAGG4560.18188998049469288No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAA4490.17909780974148487No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCATCACTTT4370.17431123130741402No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG4150.16553583751161743No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTA4100.16354142983075456No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCAAT4060.16194590368606426No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGGTTT4050.1615470221498917No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGAAG3980.1587548513966837No Hit
GATGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG3950.15755820678816598No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCT3890.15516491757113055No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTGCTT3820.15237274681792257No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG3690.1471872868476791No Hit
GATGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGAAGCACCTC3630.14479399763064368No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATCTT3630.14479399763064368No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA3460.13801301151570997No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCATCACTTC3350.13362531461781166No Hit
GATGAACGCTGGCGGGGTGCCTAATACATGCAAGTCGGACGCAATGCTTC3350.13362531461781166No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGCCTT3350.13362531461781166No Hit
GATAAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTTTTC3300.1316309069369488No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTCTCTTC3250.12963649925608595No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGT3190.12724321003905048No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTG3160.1260465654305328No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA3120.12445103928584249No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG3060.12205775006880706No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATCCCAG3040.12125998699646191No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCACCCC2990.11926557931559907No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCCA2890.11527676395387335No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAGCGAT2870.1144790008815282No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGAATGCA2800.1116868301283202No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC2800.1116868301283202No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAACATAA2770.11049018551980247No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCATGG2760.1100913039836299No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC2700.10769801476659448No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA2610.10410808094104132No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAAGCACC2570.10251255479635103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAACC206.175287E-8294.99997295
CGCTGGC165750.0294.466067
TGAACGC152250.0294.41873
CGCTAGC84600.0294.302587
ACGCTAG84600.0294.302586
ACGCTGG166500.0293.4946
CTGGCGG165850.0293.310249
CTAGCTA36750.0292.99329
CTAGCGA23900.0292.53149
CTAGCGG24350.0291.971259
GCTAGCG48350.0291.644268
ACGAACG94700.0291.417662
GCTGGCG167950.0290.433178
AACGCTG168450.0290.00895
ATGAACG152500.0288.036072
GATGAAC152600.0287.074041
ATTGAAC2850.0284.64911
GACGAAC83300.0283.844541
ATAAACG4650.0282.311862
CAGACAC5850.0279.8718295
Produced by FastQC (version 0.11.5)