FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169710
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG112036.601260974603735No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT106986.303694537740852No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG81084.777561722939132No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG65563.86306051499617No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAAG45642.6892935006776266No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC43942.5891226209416063No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT43592.568499204525367No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC43472.561428318896942No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT43062.53726945966649No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG35372.0841435389782568No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA33921.998703670968122No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT32671.9250486123386954No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGAAGAT31171.8366625419833835No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT28511.6799245772199636No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGA28321.6687290083082906No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA26031.5337929408991808No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATTACA25661.5119910435448707No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT24891.4666195274291438No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCACC21621.2739378940545638No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG21541.2692239703022805No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCATA20771.2238524541865536No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG20651.2167815685581287No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT18581.094808791467798No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC17691.0423663897236461No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA13450.792528430852631No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGTC12400.7306581816039126No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC11960.7047316009663543No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA10770.6346119851511403No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTTTT10750.6334335042130694No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAATCTTCTT10670.628719580460786No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGTTAAGGCG10190.6004360379470862No Hit
GATAAACGCTGGCGGCGTGCCTAATACATGCAAGTCGATCGATAGAAGTC10180.5998467974780508No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG8680.5114607271227388No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATCTTA8550.5038006010252785No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGGAGAAGCCT8140.47964174179482644No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT7070.4165930116080373No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATTTT7020.4136468092628602No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGATGCAT6980.41128984738671853No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGAAGAAGATC6780.3995050380060102No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT6620.3900771905014437No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG6500.3830063048730187No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGAGA6170.36356136939485006No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA5870.34588415532378763No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT5800.34175947204053975No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTAAGACG5790.34117023157150433No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA5510.3246714984385128No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT5510.3246714984385128No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC5410.3187790937481586No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT5290.31170820811973365No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATCT5090.2999233987390254No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA5040.29697719639384834No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC4910.2893170702963879No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGCGGCCC4740.2792999823227859No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC4590.2704613752872547No Hit
GATGAACGCTGGCGGCGCACTTAAGACATGCAAGTCGAACGGACTTAACC4550.2681044134111131No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG4260.25101643980908606No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAAGAGG3930.23157150433091742No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGGGACCT3890.2292145424547758No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGAT3890.2292145424547758No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGACCATA3730.21978669495020917No Hit
GACAAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGACGGAGGC3670.2162512521359967No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA3600.21212656885274883No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG3570.21035884744564254No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT3430.20210948087914676No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT3420.20152024041011135No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG3400.20034175947204053No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATC3320.19562783571975723No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCATGG3310.19503859525072184No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA3200.18855695009133228No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT3160.18619998821519063No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA3130.18443226680808436No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGGGACCC3110.18325378587001354No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGAAGCTGCTT2930.1726474574273761No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAAGTATTT2890.17029049555123446No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGGGTTATAC2890.17029049555123446No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT2860.16852277414412822No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAATTTCG2810.16557657179895116No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAA2770.16321960992280948No Hit
GATGAACGCTGGCGGCGTGCATAACACATTCAAGTCGAACGGACGACTCG2770.16321960992280948No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAATGTC2730.16086264804666786No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC2720.16027340757763245No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTGT2640.15555948382534912No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGTGAGGC2620.1543810028872783No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT2520.14848859819692417No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGAGATAAAG2470.1455423958517471No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAACGCCAA2400.14141771256849922No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGA2400.14141771256849922No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATCTT2380.14023923163042837No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG2360.13906075069235754No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC2340.13788226975428672No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT2320.1367037888162159No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT2270.13375758647103883No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAACCTT2260.13316834600200342No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTCTTTT2220.13081138412586177No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2160.1272759413116493No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGCCC2100.1237404984974368No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT2090.12315125802840139No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA1950.1149018914619056No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAAG1950.1149018914619056No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC1920.11313417005479937No Hit
GACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG1910.11254492958576395No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGGTCAAGG1810.10665252489540983No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGAAGAT1790.10547404395733898No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCGCCAT1780.10488480348830358No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGGGGCAGCGGGG1740.10252784161216193No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG1720.1013493606740911No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC1700.10017087973602026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAG32200.0295.06
TGAACGC84550.0294.825533
CGCTGGC137050.0294.784737
CGCTAGC32300.0294.08677
CTGGCGG137100.0294.031749
ACGCTGG137650.0293.928476
AACGCTG137600.0293.928045
ATAAACG4850.0291.958772
GCTGGCG138250.0291.799268
GAACGCT165600.0291.34814
GCTAGCG19450.0291.208228
CTAGCGA3250.0290.461559
TAAACGC4900.0288.979583
ACGAACG80950.0288.804842
AACGCTA32950.0288.28535
CTAGCTA13100.0288.24439
GACGAAC78850.0287.70451
AAACGCT5800.0287.37074
CGAACGC82200.0284.592473
GATAAAC5050.0280.396061
Produced by FastQC (version 0.11.5)