FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005103692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191957
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG2320312.087602952744625No Hit
ATTGAACGCTGGCGGCAGGCCTAATACATGCAAGTCGAACGGTAACATAA1945910.137166136165913No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA179059.327609829284683No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA111265.796089749266763No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGGTGAAGAGA57332.9866063753861543No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA52912.756346473428945No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT44692.3281255697890675No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA42242.200492818704189No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGG38592.0103460670879416No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA35611.855102965768375No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC31651.6488067640148576No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG30341.5805623134347797No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAATCTTCTT28881.5045036127882807No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGTTA28331.4758513625447367No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAAG28231.4706418625004558No Hit
GATAAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGCCCC27981.457618112389754No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT26341.3721823116635496No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA19641.0231458086967393No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG17530.9132253577624155No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT16310.8496694572221904No Hit
AGTGAACGCTGGCGGCAGGCCTAATACATGCAAGTCGAACGGTAACATAA14710.7663174565136984No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC14610.7611079564694176No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG14360.7480842063587158No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGG14030.7308928562125893No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT13740.7157853060841751No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA13040.6793188057742099No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTT11410.5944039550524336No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGT10560.5501232046760473No Hit
GATGAACGCTGGCGGCGCATTTAAGACATGCAAGTCGAACGAAAATTTAC9960.5188662044103627No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG9770.5089681543262293No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAGCGGAGATTGAG9520.49594440421552743No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG9250.4818787540959694No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA8750.4558312538745657No Hit
GACGAACGCTGGCGGCAGGCCTAATACATGCAAGTCGAACGGTAACATAA8170.4256161536177373No Hit
GATGAACGCTGGCGGCAGGCCTAATACATGCAAGTCGAACGGTAACATAA7840.4084248034716108No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGATG7230.37664685320149827No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC6610.3443479529269576No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT6440.33549180285168034No Hit
GATGAACGCTAGCTATAGGCTTAACACATGCAAGTCGAGGGGAAACGACA6370.33184515282068383No Hit
AATGAACGCTGGCGGCAGGCCTAATACATGCAAGTCGAACGGTAACATAA5880.30631860260370813No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT5270.27454065233359554No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC5240.2729778023203113No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA5000.2604750022140375No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAACGAAAAGAGGG4650.2422417520590549No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGGGACCC4510.23494845199706185No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG4420.23025990195720916No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGG4210.2193199518642196No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG4090.2130685518110827No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT4070.21202665180222652No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTGA3690.1922305516339597No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT3370.17556015149226128No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA3200.166704001416984No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG3160.16462020139927172No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGATCAGG3120.16253640138155942No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG3050.15888975135056288No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCCCC3020.15732690133727867No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG2880.15003360127528562No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG2850.14847075126200138No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGGAGAACATT2850.14847075126200138No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTAACATAA2700.14065650119558026No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA2660.13857270117786796No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCAGGA2560.1333632011335872No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACTTGT2470.12867465109373452No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC2400.125028001062738No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC2310.12033945102288533No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT2300.11981850101845726No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT2230.11617185098746073No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT2190.11408805096974842No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGA2190.11408805096974842No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG2120.11044140093875192No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC2110.10992045093432383No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAATCTTCTT2110.10992045093432383No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGT2090.10887855092546767No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGATA2010.10471095089004308No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC1980.10314810087675885No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGAGACCG1960.1021062008679027No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGCCAATT1950.10158525086347463No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAAAGAGGG1950.10158525086347463No Hit
GACGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA1920.10002240085019042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)