FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199376
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG180259.040707005858279No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA136086.825294920150871No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG136036.822787095738704No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA130296.534888853222053No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA109445.4891260733488485No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGGAGAA106715.352198860444587No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA76873.8555292512639436No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT58632.940674905705802No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA50352.5253791830511196No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT37991.9054449883636946No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT31221.5658855629564241No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCAAAGAG28061.4073910601075355No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA27341.3712783885723456No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT25461.2769841906749058No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG23761.191718160661263No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG23331.170150870716636No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT21791.0929098788219243No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT17400.8727228954337533No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG14850.7448238504132895No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA14660.7352941176470588No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGCTT14600.7322847283524596No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA14520.7282722092929942No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG12770.6404983548671856No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC11800.5918465612711661No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG11260.5647620576197737No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA11070.555232324853543No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA11000.5517213706765107No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC10420.5226306074953856No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAGAA9760.489527325254795No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG9750.48902576037236173No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCGGGA9690.4860163710777626No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG9350.4689631650750341No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG9160.4594334323088035No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAATGAAGTT8930.44789744001284004No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC8760.4393708370114758No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT8700.4363614477168766No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTGTGT8660.4343551881871439No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAAACTG8360.41930824171414816No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCAGCA7710.3867065243559907No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAT7670.38470026482625796No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTAAGACG7200.36112671535189794No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGGTTTGG7130.3576157611748656No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG6860.34407350934916936No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT6660.3340422117005056No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGGACGAACTTAATC6360.3189952652275098No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT6290.31548431105047753No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTGAGAGA6260.31397961640317795No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATC6220.31197335687344513No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGA6140.3079608378139796No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC5250.26332156327742556No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC5110.2562996549233609No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGGAGAA5090.2552965251584945No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGG5000.25078244121659576No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCACAG4930.24727148703956345No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAATCAAGA4920.24676992215713023No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA4850.2432589679800979No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTCTCTTC4320.21667602921113874No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAGAAAA4110.20614316668004173No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA3920.1966134339138111No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT3840.19260091485434555No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA3750.18808683091244685No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAACATAA3590.18006179279351578No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT3570.1790586630286494No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACATT3550.178055533263783No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGCGGAGACAGTG3480.17454457908675067No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT3390.17003049514485194No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA3320.1665195409678196No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAGCGGAGACAGTG3300.1655164112029532No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA3210.16100232726105448No Hit
GATAAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTTTTC3150.15799293796645533No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGAT3110.15598667843672256No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA3100.15548511355428937No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGATA3070.1539804189069898No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGGAGAA3010.15097102961239067No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATACTT3000.15046946472995745No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAATTT2990.1499678998475243No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT2830.14194286172859322No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAACGAGAGGAGAA2810.14093973196372683No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAT2770.13893347243399407No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTTACTTC2730.1369272129042613No Hit
GACGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2500.12539122060829788No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTCT2480.12438809084343151No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT2480.12438809084343151No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT2460.12338496107856511No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATCTTCTTC2240.1123505336650349No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAACATTT2220.11134740390016853No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATACT2220.11134740390016853No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAAGACG2210.11084583901773534No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC2200.11034427413530214No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGTTT2130.1068333199582698No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT2070.10382393066367065No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGTGAATCT2020.1013161062515047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACTC800.0295.0295
CACGCTA108.4597885E-4295.05
ACGCTAG50150.0295.06
CGCACGC108.4597885E-4295.03
CCTGCAA108.4597885E-4295.0295
ACGCTGG149000.0294.7036
CGCTGGC148900.0294.60387
CGCTAGC50250.0294.412937
CTAGCTA46350.0294.363569
TGAACGC98650.0294.102873
CTGGCGG149550.0292.830179
GCTAGCT47000.0290.92028
ATGAACG96900.0287.693482
AACGCTG153150.0286.524665
ACGAACG100850.0285.639562
GACGAAC100500.0285.606961
GCTGGCG154100.0284.471138
ATAAACG2650.0283.867922
GAACGCT205550.0281.437624
CGAACGC102500.0281.185363
Produced by FastQC (version 0.11.5)