FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121069
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT1757014.512385499178155No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1748514.44217760120262No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1457012.034459688276932No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT86757.165335469856032No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGCCGGCT35892.9644252451081616No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT32372.6736819499624183No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT28742.3738529268433703No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT28562.3589853719779628No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTTCGGCT27812.2970372267054326No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT21811.8014520645251881No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT21681.7907143860112829No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT19321.5957842222203866No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT18111.4958412145140374No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCTGATCATCCTCTCAGACCAGCT16181.3364279873460587No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT15051.2430927818021127No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT14851.2265732763961046No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT12291.0151236071992005No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT12181.006037879225896No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT10430.8614922069233246No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGGTCTCTCAACCCGGCT8560.7070348313771486No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT7900.6525204635373217No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT7650.6318710817798114No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT7590.6269152301580091No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT7430.6136996258332026No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT7300.6029619473192973No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT6710.5542294063715733No Hit
TTGGACCGTATCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT6220.5137566181268532No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT6120.5054968654238492No Hit
TTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT5690.4699799288009317No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT5660.4675020029900305No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT5390.4452006706919195No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGTCCGATCAGCCTCTCAGCTCGGAT5080.41959543731260684No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT4870.4022499566362983No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT4350.35929924258067714No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCGGTCTCTCAACCCGGCT4220.34856156406677186No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT4070.3361719350122657No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGTTTTGCCTCTCAGCACAGCT4000.3303901081201629No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT3710.30643682528145105No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGTTCAACCTCTCAGTCCGGCT3570.29487317149724535No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT3080.2544003832525254No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT3030.25027050690102337No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGCCGGCT3000.24779258109012217No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2850.23540295203561606No Hit
CTGAACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2590.21392759500780548No Hit
CTGGACCGTCTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT2530.20897174338600305No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2520.20814576811570262No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT2450.20236394122359977No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2420.19988601541269854No Hit
TTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2330.1924522379799949No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATTAACCTCTCAGTTCGGCT2180.18006260892548875No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2180.18006260892548875No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2080.1718028562224847No Hit
CTGGACCGTGTTTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT2030.16767297987098267No Hit
CTGGGCCGTATCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT1970.16271712824918022No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGGTCGGCT1950.1610651777085794No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGGCTCTCAACCCGGCT1900.15693530135707737No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGCCGGCT1870.15445737554617617No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT1830.1511534744649745No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTACATCCTCTCAGATCGGCT1730.14289372176197046No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAAGCCGGCT1470.12141836473415987No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT1390.1148105625717566No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTTCGGCT1340.11068068622025456No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT1280.10572483459845212No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGCCACCCTCTCAGGTCGGCT1230.1015949582469501No Hit
CTGGACCGTATCTCAGTTCCAGTGTGTCCGATCAGCCTCTCAGCTCGGAT1220.10076898297664968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG17850.0295.04
TGAACCG255.293259E-10295.02
AATTACG108.4556907E-4295.0295
GTGTCTC91500.0294.51648
GGACCGT24400.0294.39553
GCCGTGT72050.0294.385835
CGTGTCT91550.0294.355537
CCGTATC24300.0293.785986
GGCCGTG72200.0293.774234
TGTCTCA91800.0293.71469
CCGTGTC91700.0293.713176
TGGACCG24500.0293.193882
CGTATCT24350.0293.182747
GTATCTC24350.0293.182748
GCCGTAT22550.0292.38365
GGCCGTA22600.0291.736724
TTGGACC17500.0291.628571
ACCGTGT18100.0290.925385
TTGGGCC19450.0289.691531
CTGGACC7150.0288.81121
Produced by FastQC (version 0.11.5)