FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209626
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA2824513.473996546229952No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGGACAA123615.89669220421131No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG103864.954538082108136No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATTACT80193.8253842557697997No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT50982.4319502351807505No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG47942.2869300563861352No Hit
GATGAACGCTAGCTACAGGCCTAACACATGCAAGTCGAGGGGAAACGGCA44952.144295077900642No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT43482.0741701888124564No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA40051.9105454476066899No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA38441.8337419976529628No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA34851.6624846154580062No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC34631.6519897340978695No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC33281.5875893257515767No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA32451.5479950006201522No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC31951.5241429975289325No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT29391.4020207417018882No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGG27481.3109060898934293No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA26601.2689265644528829No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT25171.2007098356119947No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC24131.1510976691822579No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGAAAGTT23821.1363094272657017No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG20320.9693454056271646No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC19460.9283199603102669No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA17160.8186007460906567No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG16670.7952257830612615No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA16170.7713737799700419No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA15980.7623100187953784No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA15450.7370268955186857No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT15260.7279631343440223No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT14300.6821672884088805No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAGTTAGA14270.6807361682234074No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAG13930.6645168061213781No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGTGAATCT11310.5395323099233873No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATTACA10830.5166343869558165No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTTACA9610.4584354994132407No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC9250.44126205718756256No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC9140.4360146165074943No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG9100.4341064562601967No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGGACAA9080.43315237613654795No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAAA8410.4011906919943136No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTG8170.3897417305105283No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT8160.3892646904487039No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGATTT7410.3534866858118745No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT7320.34919332525545493No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAT7040.33583620352437193No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGT6920.33011172278247924No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAAG6570.31341532061862554No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTTAAGGCG6510.3105530802476792No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT6420.30625971969125965No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT6410.3057826796294353No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG5740.273820995487201No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTATGT5730.27334395542537665No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA4860.2318414700466545No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC4650.22182362874834227No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCTTACGCGAAAGGGA4450.21228282751185445No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC4290.20465018652266417No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAATTT4270.20369610639901536No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT4220.20131090608989344No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA4180.19940274584259585No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA4080.19463234522435194No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAACGAGAGGACAA3950.18843082442063483No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG3930.18747674429698605No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAATCT3900.18604562411151288No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC3800.18127522349326897No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA3760.1793670632459714No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT3700.17650482287502506No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG3680.17555074275137625No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGCTAGGA3650.1741196225659031No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA3610.1722114623186055No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA3370.1607625008348201No Hit
GATGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATTACT3280.1564691402784006No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA3280.1564691402784006No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGTTT3220.15360689990745424No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGGCA3120.1488364992892103No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAATCAAGA3070.14645129898008835No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT2950.14072681823819566No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA2920.13929569805272246No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAATTT2890.1378645778672493No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA2850.13595641761995172No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGG2850.13595641761995172No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG2710.12927785675441023No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT2650.12641561638346388No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG2620.12498449619799071No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAATTTACA2570.12259929588886874No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG2400.11448961483785408No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCATTTT2380.1135355347142053No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGGAGAACATT2340.11162737446690774No Hit
GATGAACGCTAGCTACAGGCCTAACACATGCAAGTCGAGGGGAAACGACA2340.11162737446690774No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA2320.11067329434325894No Hit
AGTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGGACGCGAAAGGGA2230.10637993378683941No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA2220.10590289372501503No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTTGAACGGACTTAATC2200.10494881360136625No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCATTGA2200.10494881360136625No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTAAGA2130.1016095331685955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGC136500.0294.891947
CGCTAGC72700.0294.594247
CTAGCTA53700.0294.450659
ACGCTAG72650.0294.390936
TGAACGC130750.0294.323123
ACGCTGG137050.0294.03146
CTGGCGG136800.0293.813969
AACGCTG137200.0293.602425
GCTGGCG137000.0293.49278
AAACGCT10900.0290.940374
ATAAACG10750.0289.511632
GAACGCT202250.0289.09274
ACGAACG69300.0287.763342
ATTGAAC6050.0287.685941
GATAAAC10850.0286.843321
TAAACGC10850.0286.843323
GCTAGCT55450.0285.689828
AACGCTA75000.0284.576665
GACGAAC68800.0284.49491
CGAACGC70300.0283.87983
Produced by FastQC (version 0.11.5)