FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005103705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209376
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2384111.38669188445667No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT189639.05691196698762No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT180558.623242396454225No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT178318.51625783279841No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT160107.6465306434357325No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT147127.026593305823018No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT111785.338720770288859No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT96244.596515359926639No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT60002.865657955066483No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT37331.7829168577105303No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTTAACCTCTCAGTCCGGCT36541.7451856946354882No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT32781.5656044627846553No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT22701.0841739263334862No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT20170.9633386825615162No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT19660.938980589943451No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT18810.8983837689133425No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT18540.8854883081155434No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT18020.8606526058383005No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT17450.8334288552651689No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT14820.7078175149014214No Hit
CTGATCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT14010.6691311325080238No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT13480.6438178205716032No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT12930.6175492893168272No Hit
CTGGACCGTGTTTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT9630.4599381017881705No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT9310.4446545926944826No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT8750.41790845178052877No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT7240.345789393244689No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT7090.3386252483570228No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT6850.3271626165367568No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT6620.31617759437566867No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT6400.3056701818737582No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCGGTCTCTCAACCCGGCT6200.2961179886902033No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT5780.2760583830047379No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT5510.2631629222069387No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT5130.24501375515818433No Hit
CTGGGCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT5090.24310331652147332No Hit
TTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT4900.23402873299709614No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGCCCTCTCAGGCCGGCT4760.2273421977686077No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT4090.1953423506036986No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTTCGGCT3960.1891334250343879No Hit
CTGGGCCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT3810.1819692801467217No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT3670.1752827449182332No Hit
TTGGGCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT3610.17241708696316674No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3610.17241708696316674No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGTCCGGCT3430.1638201130979673No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT3420.16334250343878956No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGGTCTCTCAACCCGGCT3330.15904401650618982No Hit
CTGGGCCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT3300.15761118752865658No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3220.1537903102552346No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTCAACCTCTCAGTCCCCCT3180.15187987161852362No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGCCGGCT3170.15140226195934586No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT3020.14423811707167966No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGTCGGCT2970.14185006877579093No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT2940.14041723979825768No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2940.14041723979825768No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTTCGGCT2790.13325309491059148No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT2790.13325309491059148No Hit
TTGGACCGTTTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2730.130387436955525No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCGGCCTCTCAGCCCGGCT2720.12990982729634723No Hit
CTGGACCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT2600.12417851138621429No Hit
CTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2590.12370090172703653No Hit
TTGGGCCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT2550.12179046309032553No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2420.11558153752101483No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2370.11319348922512609No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCAACCTCTCAGTCCGGCT2330.11128305058841509No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2230.10650695399663763No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2180.10411890570074889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)