FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167172
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3225419.29390089249396No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2723916.29399660230182No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT97175.812576268753141No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT89475.351972818414567No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT73654.405642093173498No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT62613.745244418921829No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT59243.5436556361113105No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT57933.465293230923839No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT52123.117746991122915No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT42622.549470006938961No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT40392.416074462230517No Hit
CTGGACCGTGTTTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT33241.988371258344699No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT28491.7042327662527217No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT14020.8386571913956883No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT13500.807551503840356No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGTCGGCT13490.806953317541215No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT12540.7501256191228196No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT11800.7058598329863852No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT11680.6986815973966932No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT10830.6478357619697078No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT9760.5838298279616204No Hit
ATGGGCCGTGTCTCAGTCCCATTGTGGCTGATCATCCTCTCAAACCAGCT8780.5252075706458019No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT8740.5228148254492379No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT7820.4677816859282655No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTTAACCTCTCAGTCCGGCT7130.42650683128753625No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT6350.37984829995453784No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT6150.36788457397171775No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGGTCTCTCAACCCGGCT5310.31763692484387335No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT5190.31045868925418135No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTTCGGCT5110.30567319886105326No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT5100.3050750125619123No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT4710.2817457468954131No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT4690.28054937429713106No Hit
TTGGACCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT4580.27396932500658006No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT4400.26320197162204195No Hit
TTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT4370.2614074127246189No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT4070.2434618237503888No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3820.2285071662718637No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3640.21773981288732563No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGGTTAACCTCTCAGTCCCCCT3210.19201780202426244No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT3100.18543775273371138No Hit
TTGGTCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2940.1758667719474553No Hit
TTGGACCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT2770.16569760486205826No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT2720.16270667336635322No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2560.15313569258009715No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2360.14117196659727704No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT2310.13818103510157204No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT2240.133993731007585No Hit
TTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT2150.12861005431531597No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGTCGGCT2140.12801186801617495No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2140.12801186801617495No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2100.12561912281961093No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTACATCCTCTCAGATCGGCT2070.12382456392218792No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGATCTTCCTCTCAGAACCCCT2000.1196372598282009No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTCAACCTCTCAGTCCCCCT1970.11784270093077788No Hit
CTGGGCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT1920.11485176943507286No Hit
TTGGGCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT1920.11485176943507286No Hit
TTGGGCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT1870.11186083793936785No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT1760.10528078864881678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCCG27200.0295.000032
ATTAGGG255.293259E-10295.0295
CGTATCT12800.0295.07
ACCGTAT305.456968E-12295.05
GTGTCTC148650.0294.70238
CGTGTCT148600.0294.70227
CCGTGTC148850.0294.405466
TGTCTCA148900.0294.30669
TTGGACC39700.0294.256931
CTGGGCC59500.0294.25631
GGACCGT47500.0294.068423
GCCGTGT79800.0293.706155
TGGACCG47600.0293.450652
ACCGTGT47000.0293.430855
GGTCCGT27400.0293.385043
GTCCGTG27300.0293.379154
TTGGGCC31550.0293.129941
TTGGTCC21150.0292.90781
GTATCTC12900.0292.71328
CTGGTCC6250.0292.639981
Produced by FastQC (version 0.11.5)