FastQCFastQC Report
Tue 5 Jul 2022
EGAF00005103718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117843847
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2327340.19749355263325713No Hit
GTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGA1370200.11627251102893814No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG1297760.11012539330967361No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC1235900.10487607384371965No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1222060.10370163832143055No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG1212420.1028836066426107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)