FastQCFastQC Report
Sat 2 Jul 2022
EGAF00005103877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63248921
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGATCTCGGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG13034582.0608383184908403Illumina Single End PCR Primer 1 (97% over 41bp)
GAAGATCTCGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG2778370.4392754779168485Illumina Single End PCR Primer 1 (97% over 42bp)
GAAGATCTCGGCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCA819030.12949311815137526No Hit
GAAGATCTCGCGCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCC723410.11437507368702779No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)