FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005104146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275265
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT208997.592320127876773No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG202837.368535774617187No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA182126.616169872668157No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA144285.241494559787841No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT125104.5447114598659475No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG119704.348536864476051No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG102823.7353096107387427No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA82793.0076471763573287No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG63472.305778068406808No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG62692.2774417379616008No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC57652.0943454489310303No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG52961.923964179972027No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC44001.5984596661399015No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG42941.5599513196374404No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT42761.5534121664577771No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTTTT40261.46259059451801No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC39461.4335276914972845No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT32781.1908524512742267No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACTTTATA32621.1850398706700815No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG32311.1737779957495504No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC30391.1040270284998093No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG30281.1000308793344595No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA26540.9641618077125679No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG23050.837374893284653No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA22130.8039525548108186No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA19770.7182169908996785No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT18800.6829782209870489No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT17730.6441065881968285No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA15120.5492888670917117No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC14720.5347574155813489No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG13940.5064210851361415No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG12920.46936588378471655No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC12110.43993969447623205No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG11740.4264981018291465No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT10800.392349190779794No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA10660.38726318275116706No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG10520.3821771747225401No Hit
GATAAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTTTTC10300.37418487639184056No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT8870.3222349372422938No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT8670.31496921148711243No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT8610.312789493760558No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA8370.3040706228543404No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT8260.3000744736889906No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA8120.29498846566036363No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT7870.2859063084663869No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA7650.2779140101356874No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA6880.24994096597823917No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC6820.24776124825168475No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACTTTACA6740.2448549579496122No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT6530.23722594590667176No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG6490.23577280075563548No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA6430.23359308302908108No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT6340.23032350643924945No Hit
GACGAACGCTGGCGGCGCGCTTAACACATGCAAGTCGAACGAGAGATGAG6180.22451092583510435No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT6170.22414763954734526No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC5950.21615534121664579No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTA5830.21179590576353696No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA5560.20198717599404212No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGTTT5440.19762774054093327No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAATTTACA5390.19581130910213793No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT5140.18672915190816122No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGTAAATG5060.18382286160608866No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATTACA4870.17692042213866638No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG4730.1718344141100394No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT4260.15475995858536318No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAA4170.15149038199553158No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA4130.1500372368444953No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA4130.1500372368444953No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT4120.14967395055673624No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC4110.14931066426897718No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT4060.14749423283018182No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACATT4020.14604108767914556No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGGATTTAA3980.14458794252810928No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATACT3920.14240822480155485No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACATC3900.14168165222603674No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC3820.13877536192396417No Hit
GACGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG3500.12715020071567398No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA3350.12170090639928796No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT3240.11770475723393821No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC3230.11734147094617914No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAAA3180.1155250395073838No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGAACAGG3160.11479846693186566No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC3080.1118921766297931No Hit
GACGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG3060.11116560405427496No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATTT3020.1097124589032387No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA2970.10789602746444336No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA2950.10716945488892522No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGTATTTT2940.10680616860116615No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC2900.10535302345012988No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATACTT2900.10535302345012988No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAATCT2900.10535302345012988No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAAGACG2870.10426316458685267No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAT2830.1028100194358164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)