FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315139
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA3252910.322111830017864No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA224087.1105131386467555No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT207936.598040864507408No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC205096.507921901129343No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT141944.504044247141738No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC99033.142422867369637No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG96093.0491307010557245No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA67562.143815903458474No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT61051.937240392334811No Hit
ATTGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAACGGCAGCGGGG53611.7011540939077676No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAATTTACA49501.5707354532444415No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT48131.5272625730233325No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT46431.4733181231139276No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG46141.4641158345999703No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGTTTA43721.3873243235524642No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT41491.316561898083068No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT41231.308311570449865No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATACTT40731.2924455557706283No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC32741.0389066411964245No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC28530.9053147975972506No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC25820.8193209980357875No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG21940.69620072412491No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT20700.6568530077204028No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGG20420.6479680395000301No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA14870.47185527656050186No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG14760.46836475333106975No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC14610.46360494892729875No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG14390.45662390246843454No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT14000.44424841101862983No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT13780.43726736455976567No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAAGGGCATCGGGG13570.43060363839448623No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAGCGGAGATTGAG13470.42743043545863885No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA13060.4144203034216647No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAGTCAT13030.4134683425409106No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT12910.40966049901789375No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG12770.4052180149077074No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACAG12670.4020448119718601No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCATCACTTC12180.386496117586208No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACACTT10910.3461964403009466No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATGAAACCG10740.34080199531000605No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC9880.3135124500617188No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA9710.3081180050707783No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT9560.30335820066700725No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG9460.30018499773115986No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC9450.29986767743757514No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT9310.29542519332738887No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGGTTTGG9260.29383859185946515No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC9150.2903480686300331No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC9130.28971342804286365No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG8810.2795591786481521No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC8800.2792418583545674No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA8240.2614719219138222No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT8180.25956800015231374No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAATTTCA7920.25131767251911064No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG7910.25100035222552586No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCATCACTTT7870.24973107105118694No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGAGG7750.2459232275281701No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAAGGGCAGCGGGG7600.24116342312439906No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAAGGGCATCGGGA7460.23672093901421276No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC6870.21799904169271336No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA6360.20181570671989188No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC6250.19832518349045977No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA5850.18563237174707034No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCATCACCTC5840.18531505145348562No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA5460.17325688029726566No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTATAT5290.16786243530632514No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC5110.1621506700217999No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA5060.16056406855387623No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA5010.15897746708595253No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCAAT4760.15104445974633415No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT4670.14818857710407152No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGT4610.1462846553425631No Hit
GATGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAACGGCAGCGGGG4260.1351784450670974No Hit
GACGAACGCTGGCGGCGTGCTTCATACATGCAAGTCGAACGAGAAGTTAC4070.1291493594889874No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG4020.12756275802106373No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT4000.12692811743389426No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT3890.12343759420446215No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGATTT3860.12248563332370795No Hit
GACGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA3850.12216831303012321No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA3780.11994707097503006No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCATTTT3760.11931243038786059No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGTTTA3710.11772582891993691No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATTT3680.1167738680391827No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTT3640.11550458686484376No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGAGATAAAT3590.11391798539692008No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTTAGA3490.11074478246107272No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA3420.10852354040597957No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG3420.10852354040597957No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGGTTA3360.10661961864447116No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGACTTAAAA3260.10344641570862383No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTG3230.10249445482786962No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC3220.10217713453428488No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGT3210.10185981424070015No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACC108.462116E-4295.0295
TGAACGC197100.0294.476173
CTGGCGG236750.0294.439279
ACGCTAG77550.0294.42946
ACGCTGG237500.0294.378976
CGCTAGC77600.0294.23977
AACGCTG238700.0292.899055
CTAGCTA44900.0292.043439
GCTAGCG33000.0291.871228
CGCTGGC239500.0291.674357
GCTGGCG240700.0290.036358
GCTAGCT45900.0287.28768
ACGAACG112600.0286.223362
GAACGCT315700.0285.982764
GACGAAC111850.0284.977631
AACGCTA80600.0283.470835
ATGAACG198050.0280.9242
GATGAAC198850.0279.20041
ATAAACG8300.0279.006042
GATAAAC8350.0277.335331
Produced by FastQC (version 0.11.5)