FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188628
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA146037.741692643722035No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG144317.650507877939648No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA112935.986916046398202No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA112535.965710286913926No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT98665.230400576796658No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA84234.465402803401404No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA75263.989863646966516No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC73523.897618593209916No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAAGAGAT67803.59437623258477No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAACATGG52822.8002205398986364No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA47582.5224250906546217No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA35001.8555039548741437No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT32861.7420531416332676No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGG32531.7245583900587398No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC30431.613228152766291No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC29931.5867209534109465No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGATTT24381.292491040566618No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC23851.2643934092499522No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT21901.1610153317641072No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA21381.1334478444345484No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG21241.126025828615052No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT19831.0512755264329794No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA14570.7724197892147507No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAATTT13220.7008503509553194No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG11020.5842186737918018No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC10470.5550607545009224No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAAGACAT10330.5476387386814259No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA10080.5343851390037535No Hit
AATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA9490.5031066437644464No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA9010.4776597323833153No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC8660.45910469283457384No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT8060.4272960536081599No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG7950.4214644697499841No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA7850.41616302987891507No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA7750.4108615900078461No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA7400.3923065504591047No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT6710.3557266153487287No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG5930.31437538435439066No Hit
AATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA5320.28203660114086987No Hit
AGTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC5250.27832559323112155No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC5210.276205017282694No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA5050.26772271348898363No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT4770.25287868184999046No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA4630.24545666603049388No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA4520.23962508217231798No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCACAG4170.22107004262357657No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT4140.21947961066225585No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG4100.21735903471382828No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA4090.21682889072672135No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGAT4070.2157686027525076No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTAAGACG3980.2109973068685455No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATACTT3980.2109973068685455No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGAAAGTT3930.208346586933011No Hit
AATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC3880.20569586699747652No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA3860.20463557902326274No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC3590.19032169137137647No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT3480.18449010751320058No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTATTT3400.1802489556163454No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG2990.15851305214496259No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA2980.15798290815785568No Hit
GATGAACGCTGACAGAATGCTTAACACATGCAAGTCAACTTGAATTTGGG2900.1537417562610005No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA2810.1489704603770384No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAATTTACA2800.14844031638993152No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGGAGAACATT2610.13836758063490043No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAAGAGAT2530.13412642873804526No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT2520.13359628475093838No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA2450.12988527684119006No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACATT2440.12935513285408318No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACGATGAA2420.12829484487986936No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGG2350.12458383697012108No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCGGGA2240.1187522531119452No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT2190.11610153317641071No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTA2130.11292066925376933No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT2120.11239052526666243No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA2110.11186038127955551No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT2030.10761922938270034No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2010.10655894140848654No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT2000.10602879742137965No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATACT1980.10496850944716585No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAACATGG1890.10019721356320375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT206.171285E-8295.0295
CTCAGAC108.4594276E-4295.0295
GTCCTGG157.2307903E-6294.99997295
CTGAACC157.2307903E-6294.99997295
TGAACGC161600.0294.726173
CGCTAGC77900.0294.62137
ACGCTAG77950.0294.432346
AACGCTG110500.0294.19915
CGCTGGC110400.0294.19847
ACGCTGG110350.0294.198036
ATTGAAC35000.0293.735721
GCTAGCG23250.0293.731178
CTGGCGG110450.0293.5319
CTAGCTA54800.0293.115889
AACGCTA78750.0291.441285
CTAGCGA11800.0288.750039
ACGAACG25800.0288.139532
GCTAGCT55950.0287.618448
GAACGCT191750.0287.230774
GCTGGCG113650.0285.395978
Produced by FastQC (version 0.11.5)