FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005104158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199920
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG190699.538315326130451No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA142477.1263505402160865No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC66063.3043217286914763No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG59142.9581832733093236No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG52432.622549019607843No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG47072.354441776710684No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT44602.230892356942777No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG44372.2193877551020407No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT43092.155362144857943No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG42452.1233493397358942No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA39041.9527811124449779No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC38591.9302721088435375No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC38401.9207683073229291No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT36471.8242296918767509No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA35761.7887154861944778No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG35401.7707082833133252No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG34081.7046818727490995No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG29781.489595838335334No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT28121.40656262505002No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG27891.3950580232092837No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC24451.2229891956782712No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT22611.130952380952381No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT22151.1079431772709083No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT21361.0684273709483794No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC18090.9048619447779112No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT17720.8863545418167267No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA16860.8433373349339736No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA16620.8313325330132053No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT14460.7232893157262905No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG13980.6992797118847539No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG12610.6307523009203682No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCATTTT10490.5247098839535814No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATACTT10460.5232092837134854No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC10220.511204481792717No Hit
GACGAACGCTGGCGGCGCACCTAACACATGCAAGTCGAACGAGAGAGAAG10070.5037014805922368No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAAATC9010.45068027210884354No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT8820.4411764705882353No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAATTT8370.4186674669867947No Hit
GATTAACGCTGGCGGCGTGCATAACACATTCAAGTCGAACGGAGAGATAT8090.4046618647458983No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGACTTAAAA7740.3871548619447779No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCAAT7710.38565426170468187No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCAGGA7520.3761504601840736No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA7450.37264905962384953No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAGCGGAGATTGAG7450.37264905962384953No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAT6950.3476390556222489No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTAAGA6870.34363745498199283No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC6760.33813525410164064No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG6600.33013205282112845No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA5860.2931172468987595No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAATTT5730.28661464585834334No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAATATT5710.2856142456982793No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTA5670.28361344537815125No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGTGG5400.27010804321728693No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGATTT5040.25210084033613445No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGTTTT4840.24209683873549417No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGTAAATG4750.2375950380152061No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCGCGG4740.23709483793517408No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT4700.23509403761504602No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC4610.23059223689475788No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG4470.22358943577430973No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGCTAGGA4390.2195878351340536No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT4340.21708683473389356No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA4290.21458583433373346No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCACC4280.21408563425370147No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT4070.20358143257302921No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA4020.20108043217286917No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGATTT4020.20108043217286917No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTA3960.19807923169267708No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC3920.19607843137254902No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA3690.18457382953181273No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT3610.18057222889155664No Hit
GATAAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTTTTC3420.17106842737094838No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT3400.17006802721088435No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT3390.16956782713085233No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGATTTTTCA3270.16356542617046818No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGATA3180.15906362545018007No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGGGCAGCA3120.15606242496998798No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTATTT3050.1525610244097639No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT3020.15106042416966786No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG3000.15006002400960386No Hit
GATGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG2900.1450580232092837No Hit
GATTAACGCTGGCGGCGTGCATAACACATTCAAGTCGAACGGAGAGATAC2820.14105642256902762No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGGTCAAGG2790.13955582232893157No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG2750.1375550220088035No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCTCA2730.13655462184873948No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTAAGACG2700.13505402160864347No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT2660.1330532212885154No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA2640.13205282112845138No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG2580.12905162064825929No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT2500.1250500200080032No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT2490.12454981992797119No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATGC2420.12104841936774709No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTGTGT2320.11604641856742698No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC2260.11304521808723489No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGGTTT2250.11254501800720287No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACTTTATA2230.11154461784713884No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGATTT2230.11154461784713884No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTA2200.11004401760704283No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT2200.11004401760704283No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGTATTTT2130.10654261704681872No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCTCA2120.1060424169667867No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCTCT2110.10554221688675469No Hit
GACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG2070.10354141656662665No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACTTTACA2060.10304121648659463No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCAGC2040.10204081632653061No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAGTCAT2020.1010404161664666No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG2010.10054021608643458No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)