FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005104165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155277
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3475322.38129278643972No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1590510.242985116920085No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT109617.0589977910443915No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT109367.042897531508208No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT96126.190227786471918No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT69384.468144026481706No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT62744.0405211332006665No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT61783.978696136581722No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTTCGGCT41182.652034750800183No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT27201.751708237536789No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT27041.7414040714336314No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT21691.3968585173592998No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT20051.2912408148019345No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTAGAGTCTCTCAACTCGGCT19861.279004617554435No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT17351.1173580118111504No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT13290.8558897969435267No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT12250.7889127172730024No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT8850.5699491875809038No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT8160.5255124712610367No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT6830.43985909052853933No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT6170.3973544053530143No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT5800.3735260212394624No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT5360.34518956445577903No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGCTGATCGTCCTCTCAGACCAGCT4760.3065489415689381No Hit
ATGGGCCGTGTCTCAGTCCCATTGTGGCTGATCATCCTCTCAAACCAGCT4710.30332888966170135No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT4570.29431274432143845No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT4520.2910926924142017No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT4430.2852965989811756No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT4420.28465258859972825No Hit
TTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT4370.28143253669249146No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT4200.2704843602078866No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT3990.25696014219749225No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT3760.24214790342420317No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3730.24021587227986116No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT3490.2247596231251248No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT3470.22347160236223007No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGGTCTCTCAACCCGGCT3390.21831951931065127No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCAACCTCTCAGTCCGGCT3330.21445545702196717No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT3160.20350728053736225No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACTTTCCTCTCAGAACCCCT2920.1880510313826259No Hit
CTGGGCCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT2720.1751708237536789No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2690.17323879260933686No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2690.17323879260933686No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT2440.15713853307315315No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT2370.1526304604030217No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT2250.1449023358256535No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2130.1371742112482853No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTAGAGGCTCTCAACTCGGCT2120.13653020086683798No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2060.1326661385781539No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT2060.1326661385781539No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2020.13009009705236446No Hit
CTGGGCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT1980.12751405552657508No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT1930.12429400361933834No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGATCTTCCTCTCAGAACCCCT1780.11463384789762812No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1780.11463384789762812No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT1750.11270181675328607No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT1720.11076978560894402No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT1650.10626171293881258No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT1630.10497369217591788No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGGCTCTCAACCCGGCT1600.10304166103157583No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT1560.10046561950578645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)