FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223608
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT5421124.24376587599728No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT4223618.888411863618476No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT169437.577099209330615No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT145076.487692748023327No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT131325.872777360380666No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT86313.8598797896318557No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT60642.7118886623018854No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT48932.188204357625845No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT45252.0236306393331187No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT28951.2946763979821831No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT24401.091195306071339No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT20250.9056026618010089No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT18510.8277879145647741No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT17000.7602590247218347No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT15050.6730528424743301No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGGTCTCTCAACCCGGCT14320.6404064255303925No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT14230.636381524811277No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT13210.5907659833279668No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCTGATCGTCCTCTCAGACCAGCT11780.5268147830131301No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT9910.4431862902937283No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT9720.4346892776644843No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGCCGGCCGACCTCTCAGTCCGGCT8690.38862652499016137No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT8520.38102393474294305No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACTCTCTCAAGTCGGCT7760.3470358842259669No Hit
TTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT7150.3197560015741834No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT6930.30991735537190085No Hit
TTGGACCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT6370.28487352867518156No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT6330.2830846839111302No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGGGGACCTTCCTCTCAGAACCCCT6290.2812958391470788No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT6120.27369324889986046No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT5980.26743229222568066No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT5520.24686057743908982No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT4530.2025866695288183No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGACTGGTCGTCCTCTCAGACCAGCT4490.2007978247647669No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT4380.19587850166362564No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT4260.19051196737147152No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT4040.18067332116918894No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGTCGGCT3950.17664842045007334No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT3880.17351794211298344No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGCCGGCT3740.16725698543880363No Hit
TTGGTCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3720.16636256305677793No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGATCTTCCTCTCAGAACCCCT3390.15160459375335408No Hit
CTGGGCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT2920.13058566777575042No Hit
TTGGGCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT2910.13013845658473758No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT2880.12879682301169904No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT2870.1283496118206862No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2770.12387749991055776No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2650.11851096561840363No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2470.11046116418017245No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2450.10956674179814677No Hit
TTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2440.10911953060713392No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT2420.10822510822510822No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGTCCGGCT2400.10733068584308253No Hit
TTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT2380.10643626346105685No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT2380.10643626346105685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAG108.4604754E-4295.0295
CACAGCC108.4604754E-4295.0295
TGGACCT157.232582E-6294.999972
GGACCGT67850.0294.565223
CGTGTCT201450.0294.414257
GTGTCTC201400.0294.414128
GTCCGTG22800.0294.353064
TTGGACC63000.0294.297641
CCGTGTC201850.0294.269266
ACCGTGT67400.0294.124635
GGCCGTG112650.0293.95254
TGTCTCA201950.0293.83149
TGGACCG68000.0293.698552
GACCGTG67400.0293.686954
GCCGTGT112850.0293.562265
TTGGTCC16650.0293.228241
CTGGGCC94850.0292.97841
CGTATCT20100.0292.798527
GTATCTC20150.0291.339978
TGGTCCG23100.0291.168822
Produced by FastQC (version 0.11.5)