FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179540
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2585514.400690653893284No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT1814510.106382978723403No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT137247.643979057591623No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT109646.106717166091122No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT106225.9162303664921465No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT64293.580817645093015No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT58943.2828339088782443No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT56493.1463740670602656No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT49442.7537039099922023No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT46462.5877241840258436No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT45732.5470647209535477No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT35831.9956555642196723No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT27771.5467305335858306No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT26541.478222123203743No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT26311.4654116074412387No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT23021.2821655341428093No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT16210.9028628717834466No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT15240.8488359140024506No Hit
CTGGACCGTCTCTCAGTTCCAATGTGACCGGTCAACCTCTCAGTCCGGCT14410.8026066614681965No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT13020.7251865879469757No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT11330.6310571460398797No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT10940.6093349671382422No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGTTCAACCTCTCAGTCCGGCT10540.5870558092904089No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT10270.5720173777431213No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT10240.5703464409045339No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT9960.5547510304110504No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT9560.5324718725632172No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT9190.5118636515539713No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT9030.5029519884148379No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT8750.4873565779213546No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT8270.4606215885039545No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT6380.35535256767294193No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT5740.3197059151164086No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATTAACCTCTCAGTTCGGCT5500.3063384204077086No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT5470.3046674835691211No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT5360.2985407151609669No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT5150.28684415729085444No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGTCCGATCAGCCTCTCAGCTCGGAT4610.25676729419627936No Hit
TTGGACCGTGTTTCAGTTCCAATGTGGCCGATCATCCTCTTAAATCGGCT4420.24618469421855854No Hit
CTGGGCCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT4290.23894396791801267No Hit
ATGGGCCGTGTCTCAGTCCCATTGTGGCTGATCATCCTCTCAAACCAGCT4240.23615907318703355No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT3990.22223459953213767No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT3960.22056366269355018No Hit
CTGGTCCGTATCTCAGTACCAGTGTGGGGGATAATCCTCTCAGACCCCCT3670.204411273253871No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3540.19717054695332514No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT3360.18714492592180015No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT3250.18101815751364597No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT3190.17767628383647097No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTTCGGCT3160.17600534699788348No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT2920.16263785228918345No Hit
CTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2890.16096691545059597No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT2850.15873899966581262No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2830.15762504177342096No Hit
TTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2790.1553971259886376No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT2760.15372618915005012No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATTAACCTCTCAGTTCGGCT2600.14481452601091677No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT2570.14314358917232928No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT2550.14202963127993762No Hit
CTGGACCGTTTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2500.13924473654895847No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT2400.13367494708700012No Hit
CTGGGCCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT2380.13256098919460843No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT2170.12086443132449592No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATTAACCTCTCAGTTCGGCT2140.11919349448590844No Hit
TTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2110.11752255764732092No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGTCCGGCT2090.11640859975492926No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCGTCCTCTCAGACCAGCT1930.10749693661579593No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGTCGGCT1890.10526902083101258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCATGG108.4590877E-4295.0295
GGACCGT79750.0295.03
GTGTCTC149650.0294.70438
CGTGTCT149500.0294.7047
GACCGTG74700.0294.407624
TGGACCG80000.0294.078122
TGTCTCA150150.0293.919439
GCCGTGT63750.0293.843145
CTGGACC46850.0293.740661
CCGTGTC150400.0292.84246
CTGGGCC58950.0292.748081
GGCCGTG64000.0292.69534
ACCGTGT75250.0292.059785
CGTATCT24800.0291.431467
GGCCGTA22850.0290.481384
TGGTCCG13100.0289.370242
TTGGTCC5250.0286.571441
TTGGGCC28350.0286.155181
TTGGACC34200.0285.9431
CTGGTCC8000.0285.781251
Produced by FastQC (version 0.11.5)