FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195387
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG168038.599855671052833No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT155967.982107304989585No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA81284.159949228966103No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG68633.5125161858260783No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT66313.393777477518975No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT65273.3405497806916533No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA45342.320522859760373No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA42312.1654460122730783No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG40742.0850926622549095No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATTACA36331.8593867555159758No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT35571.820489592449856No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG32941.685884936049993No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC31941.634704458331414No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT27821.4238408901308686No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC27341.3992742608259505No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA26291.3455347592214426No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG23801.2180953697021808No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAACACCCC23521.2037648359409787No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG23161.1853398639622903No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG22451.1490017247820992No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA22351.1438836770102412No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC21861.1188052429281374No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT20601.054317841002728No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC17990.9207367941572366No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGATGCAT17740.907941674727592No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT16990.8695563164386577No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA16730.8562493922318272No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC16670.8531785635687122No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT15900.8137695957254064No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC13190.6750705011080573No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG11780.6029060275248609No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACATT11380.5824338364374293No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT11290.5778275934427572No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATGA11100.5681033026762271No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGGGCAGCA11020.5640088644587409No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT10790.5522373545834677No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA10460.5353477969363366No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATACTG10430.5338123826047791No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG10070.5153874106260908No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT10000.5118047771857903No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT9230.4723958093424844No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG8970.4590888851356539No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG7920.4053493835311459No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG7220.36952304912814055No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGAG6940.35519251536693847No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAA6780.3470036389319658No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT6750.3454682246004084No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGGGAACATAC6420.32857866695327737No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA6100.31220091408333206No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT6070.31066549975177465No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGA5840.2988939898765015No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC5810.2973585755449441No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGT5670.2901933086643431No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA5320.2722801414628404No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG5200.26613848413661095No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCA5160.2640912650278678No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT5050.2584614124788241No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT4720.241571854831693No Hit
AATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG4640.23747741661420668No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA4280.2190524446355182No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG4220.2159816159724035No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA4070.20830454431461665No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG3920.2006274726568298No Hit
GATGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT3810.1949976201077861No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTGAAGA3780.19346220577622872No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA3770.19295040099904293No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA3770.19295040099904293No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATTTT3650.18680874367281344No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAGTTAGA3560.1822025006781413No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGT3540.18117889112376975No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG3530.18066708634658396No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT3510.17964347679221238No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGGTTTGG3450.17657264812909762No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGTCC3390.1735018194659829No Hit
GATGAATGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG3270.1673601621397534No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGGGA3250.16633655258538182No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACCGAATT3210.16428933347663868No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC3100.158659480927595No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA3060.15661226181885182No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT3000.15354143315573707No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG2780.1422817280576497No Hit
GATAAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTTTTC2730.13972270417172075No Hit
GATGAACGCTGGCGGCGTGCATAACACATTCAAGTCGAACGGACGATAGC2680.1371636802857918No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2680.1371636802857918No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGGATGCG2670.136651875508606No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA2670.136651875508606No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC2610.13358104684549127No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATAC2560.1310220229595623No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT2510.12846299907363334No Hit
GATGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAGCGGAGGGAGAG2440.12488036563333281No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT2300.11771509875273176No Hit
GATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGTAGCA2280.11669148919836018No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA2260.1156678796439886No Hit
GACGAACGCTGGCGGCGTGCTTAACAAATGCAAGTCGAACGAGGAATGAT2240.11464427008961703No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAAAGCAC2110.10799080798620175No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTT2110.10799080798620175No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT1980.10133734588278648No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGGGG1970.10082554110560067No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTC108.45966E-4295.03
CAAACGC108.45966E-4295.03
CTGAGCC255.293259E-10295.0295
TCTGGCG108.45966E-4295.08
CGCTGGC138600.0294.787147
ACGCTAG55600.0294.73476
TGAACGC101600.0294.709633
ACGCTGG138950.0294.36316
CTGGCGG138450.0294.25429
CGCTAGC55750.0294.20637
AACGCTG139150.0294.151985
GCTAGCG44800.0294.012278
GCTGGCG138750.0293.936958
CTAGCGA27250.0293.376139
ATAAACG25750.0293.281562
AAACGCT25950.0292.72644
TAAACGC25900.0292.15253
CTAGCTA11700.0291.217969
GATAAAC25950.0291.021181
AACGCTA56500.0290.039835
Produced by FastQC (version 0.11.5)