FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005104172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213626
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGTCC184528.637525394848941No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA181348.48866710980873No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT168937.9077453119002366No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA90224.223268703247732No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA83373.90261485025231No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC69633.259434713003099No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG67993.182665031410034No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA62332.9177160083510434No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG53082.4847162798535756No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG46012.153764054937133No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT30541.4296012657635306No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGTGGCCC29551.3832585921189366No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT28051.3130424199301585No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT27151.2709127166168912No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATCTTCTTC26381.2348684148933182No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT22861.0700944641569847No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG21501.0064318013724922No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG21360.9998782919682061No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTCTCTTC21350.9994101841536143No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC21180.9914523513055526No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA20680.96804696057596No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG18170.8505518991134038No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA17770.8318275865297295No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT16700.7817400503684008No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG16210.7588027674533999No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC16000.7489725033469709No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG14930.6988849671856422No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGG14420.6750114686414576No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG14110.6605001263891099No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA14070.6586276951307426No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATTTT12420.5813899057230861No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA11810.5528353290329828No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCGCTTC10060.47091646147940797No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT8780.4109986612116503No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG7700.36044301723572975No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG7510.35154896875848446No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT6970.3262711467705242No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA6580.30801494200144175No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT6380.29865278570960463No Hit
GACGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGTCC6120.28648198253021634No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA6090.2850776590864408No Hit
AATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA5840.2733749637216444No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACATGT5420.2537144355087864No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGGAA5220.24435227921694924No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGAAGAT5110.23920309325643885No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAAGAA5070.23733066199807137No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA4920.23030904477919353No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC4800.22469175100409125No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCGGGA4690.21954256504358086No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACGACCCG4530.2120528400101111No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATCATC4520.21158473219551927No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT4400.20596743842041698No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG4220.19754149775776356No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACATA4100.1919242039826613No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA4100.1919242039826613No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA4090.19145609616806944No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA4040.18911555709511016No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC4040.18911555709511016No Hit
AATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA3970.18583880239296716No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG3870.18115772424704857No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT3800.1778809695449056No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACATT3780.17694475391572187No Hit
GATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGTAGCA3770.17647664610113No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT3710.17366799921357887No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA3680.1722636757698033No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG3660.1713274601406196No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG3560.16664638199470103No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA3490.16336962729255802No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT3410.15962476477582316No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTCT3380.1582204413320476No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTA3290.15400747100072087No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGGTTTGG3260.15260314755694532No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT3260.15260314755694532No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGCTGATT3250.15213503974235346No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA3250.15213503974235346No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA3230.15119882411316973No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC3140.14698585378184303No Hit
GATAAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTTTTC3120.1460496381526593No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATTAAACCG2950.13809180530459778No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT2930.13715558967541402No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTATC2910.13621937404623033No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGTGAATCT2820.1320064037149036No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG2820.1320064037149036No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGATA2750.1287296490127606No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA2680.12545289431061762No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTAGAT2630.12311235523765834No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACACGG2620.12264424742306648No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT2620.12264424742306648No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATACT2580.12077181616469905No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAATTT2540.11889938490633163No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG2490.11655884583337234No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGGACGAAGTGGCCC2470.11562263020418863No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA2440.11421830676041306No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATAT2440.11421830676041306No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATTAAGACG2370.11094155205827005No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC2330.10906912079990264No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT2220.1039199348393922No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGAGG2200.10298371921020849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)