FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199828
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3662618.32876273595292No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3106615.546369878095161No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT123276.168805172448306No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT103685.188462077386553No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT97424.875192665692495No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT68703.4379566427127326No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT58612.9330223992633666No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT55982.801409211922253No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT49402.4721260283844106No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT43572.1803751226054406No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT41232.0632744159977583No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT30631.5328182236723582No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT28521.4272274155773967No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT27681.3851912644874593No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT25901.2961146586064014No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT25091.2555797986268191No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGTTTTGCCTCTCAGCACAGCT19110.9563224372960746No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT18970.9493164121144183No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT16550.8282122625457893No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT14880.744640390736033No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGCTGATCGTCCTCTCAGACCAGCT14440.7226214544508277No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT14380.7196188722301179No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT12950.6480573293032007No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT12930.6470564685629642No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT12320.6165302159857478No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT10670.533959204916228No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT10090.5049342434493664No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT9600.48041315531356965No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT8010.4008447264647597No Hit
ATGGGCCGTGTCTCAGTCCCATTGTGGCTGATCATCCTCTCAAACCAGCT7980.3993434353544048No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT7970.39884300498428643No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT7940.3973417138739316No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT6220.3112676902135837No Hit
CTGGGCCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT5580.27924014652601237No Hit
TTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT5570.2787397161558941No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT4860.24320915987749467No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACTCTCTCAAGTCGGCT4550.22769581840382727No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGGGGACCTTCCTCTCAGAACCCCT4290.21468462878075142No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCGGCCTCTCAGCCCGGCT3910.19566827471625597No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3500.17515062954140562No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT3490.1746501991712873No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT3310.16564245250915788No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3290.16464159176892126No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT3020.15112997177572712No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT2890.1446243769641892No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2630.13161318734111338No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTACATCCTCTCAGATCGGCT2610.13061232660087674No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT2600.13011189623075844No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT2490.12460716215945712No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT2460.12310587104910223No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGTCGGCT2420.12110414956862901No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGATCTTCCTCTCAGAACCCCT2300.11509898512720941No Hit
TTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT2280.1140981243869728No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2230.1115959725363813No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT2200.11009468142602638No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2150.10759252957543487No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2130.10659166883519827No Hit
TTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT2120.10609123846507998No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGGCTCTCAACCCGGCT2000.10008607402366035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC25250.0295.06
GGACCGT58050.0295.03
CGTGTCT171650.0294.742227
GACCGTG56650.0294.739624
GTGTCTC171900.0294.313578
CCGTGTC172050.0294.14276
TGTCTCA172100.0294.057229
CTGGGCC92050.0294.038571
TTGGACC40250.0293.90061
CGTATCT25350.0293.83637
GCCGTGT102900.0293.423225
TGGACCG58400.0293.232062
GCCGTAT24450.0293.19025
CTGGTCC7800.0293.108981
GTATCTC25600.0292.69538
GGCCGTG103350.0292.00294
ACCGTGT57250.0291.650635
CTGGACC18200.0290.94781
TTGGGCC34050.0290.668121
GGCCGTA24800.0289.052434
Produced by FastQC (version 0.11.5)