FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005104178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253500
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT220568.700591715976332No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA184557.280078895463511No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG128425.065877712031558No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT102944.060749506903353No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG84343.3270216962524657No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG79953.1538461538461537No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG79773.146745562130177No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT73352.893491124260355No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT51162.018145956607495No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATTACA50972.0106508875739646No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTTTT48191.9009861932938859No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAG46501.8343195266272188No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGGGATTTGTG42521.6773175542406311No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA41851.650887573964497No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC41391.632741617357002No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG40201.5857988165680472No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT30491.2027613412228797No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA23080.9104536489151873No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC22910.9037475345167653No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT22510.8879684418145956No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT20190.7964497041420118No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA19430.7664694280078895No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTTACA18160.716370808678501No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG17720.6990138067061143No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAT16530.6520710059171598No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA16130.6362919132149901No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA15570.6142011834319527No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA15150.5976331360946745No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA15110.5960552268244576No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG14060.5546351084812624No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGAT13620.5372781065088758No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC13610.5368836291913215No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGCGGGGCAGCATAA13530.5337278106508876No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGG12750.5029585798816568No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGTTACAC12440.4907297830374753No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC12110.4777120315581854No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC12000.4733727810650888No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT10780.4252465483234714No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAAAGTTC10650.42011834319526625No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTT10170.4011834319526627No Hit
GATGAACGCTGGCGGCGTGCATAACACATTCAAGTCGAACGGACGACTCG9780.3857988165680473No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA9710.38303747534516763No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA8930.35226824457593686No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGT8910.3514792899408284No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG8800.34714003944773175No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG8620.34003944773175543No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA8600.33925049309664695No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCAGCA8300.32741617357001973No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGTTAAGGCG8000.3155818540433925No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA7650.3017751479289941No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG7480.295069033530572No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCGTA7430.2930966469428008No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG7370.2907297830374753No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAA6840.26982248520710056No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG6800.2682445759368836No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT6620.2611439842209073No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC6570.2591715976331361No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA6020.23747534516765284No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACATT5950.23471400394477318No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT5810.2291913214990138No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT5700.22485207100591714No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTACTT5650.22287968441814596No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTGTGT5550.21893491124260356No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG5400.21301775147928992No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGA5220.2059171597633136No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGAGTTTAGT5070.2No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA4980.19644970414201182No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATTTT4920.1940828402366864No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA4750.18737672583826429No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCACTGATTT4690.18500986193293886No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATATTT4660.18382642998027615No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC4340.17120315581854043No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG4150.16370808678500987No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGAC3970.15660749506903351No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT3960.15621301775147928No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCACT3840.1514792899408284No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGCGCGGTTAAAGCC3700.14595660749506906No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAG3670.14477317554240632No Hit
GACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGGGATTTGTG3650.14398422090729784No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT3610.14240631163708087No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAATTACGA3540.13964497041420118No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG3530.13925049309664694No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAA3510.13846153846153844No Hit
GATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGTAGCA3370.1329388560157791No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGTTATAT3280.12938856015779093No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG3280.12938856015779093No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTGGAACGGGATTTGTG3240.12781065088757398No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC3200.12623274161735698No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTTAAGG3200.12623274161735698No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGGATTTGTG3100.1222879684418146No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA3100.1222879684418146No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAATTAAGA3060.12071005917159763No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA2970.11715976331360947No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTGCC2960.11676528599605523No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGCATAGA2960.11676528599605523No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGACACTC2900.11439842209072978No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT2830.11163708086785008No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC2800.11045364891518737No Hit
AGTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGGACGCGAAAGGGA2680.1057199211045365No Hit
AGTGAACGCTGGCGGAACGCTTGACACATGCAAGTCGAACGGTAACGCGG2620.10335305719921105No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGTC2600.10256410256410256No Hit
GATGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG2540.10019723865877712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)