FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253500
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3998615.773570019723865No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT231579.13491124260355No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT173866.858382642998027No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT159096.275739644970415No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT92073.6319526627218934No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT88223.4800788954635107No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT82503.2544378698224854No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT79573.138856015779093No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT68792.7136094674556213No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT58412.3041420118343194No Hit
CTGGACCGTGTTTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT48921.9297830374753453No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT44911.7715976331360948No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT41001.6173570019723866No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT40971.6161735700197237No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCGTCCTCTCAGACCAGCT40011.578303747534517No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT37961.4974358974358974No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT36401.435897435897436No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT28781.1353057199211045No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT27721.093491124260355No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT24920.9830374753451677No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT23540.9285996055226824No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT22220.8765285996055228No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT20070.791715976331361No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT19010.7499013806706115No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT15540.6130177514792899No Hit
CTGGTCCGTATCTCAGTACCAGTGTGGGGGGTAGTCCTCTCAGACCCCCT14830.5850098619329389No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT14630.5771203155818541No Hit
CTGGGCCGTGTTCCAGTCCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT12210.48165680473372785No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT11680.46074950690335303No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT11300.4457593688362919No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGGTCTCTCAACCCGGCT10650.42011834319526625No Hit
CTGGACCGTTTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT10370.40907297830374756No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT10220.403155818540434No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT9400.3708086785009862No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGCTGATCGTCCTCTCAGACCAGCT9040.35660749506903355No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT8750.34516765285996054No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT8050.3175542406311637No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT7920.31242603550295855No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT7860.31005917159763313No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT6880.27140039447731756No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT6030.23786982248520708No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT5960.23510848126232742No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT5930.2339250493096647No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT5910.2331360946745562No Hit
TTGGACCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT5760.2272189349112426No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT5600.22090729783037474No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT5490.2165680473372781No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGTCCACCCTCTCAGGTCGGCT5040.19881656804733727No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT4860.19171597633136095No Hit
CTGGACCGTCTCTCAGTTCCAGTGTGGCCGGTCGACCTCTCAGTCCGGCT4750.18737672583826429No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT4610.18185404339250494No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT4470.17633136094674556No Hit
TTGGTCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT4240.16725838264299803No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT4120.16252465483234713No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT4080.16094674556213018No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT4050.15976331360946747No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT3890.15345167652859962No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT3780.14911242603550295No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGACCGGCCTCTCAGCCCGGCT3540.13964497041420118No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGTTTTGCCTCTCAGCACAGCT3400.1341222879684418No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT3280.12938856015779093No Hit
CTGGGCCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT3140.12386587771203154No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCGTCCTCTCAGACCAGCT3070.12110453648915187No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2960.11676528599605523No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2950.11637080867850098No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT2930.11558185404339251No Hit
CTGGACCGTGTGTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT2870.11321499013806707No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT2760.10887573964497041No Hit
TTGGACCGTTTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGATCGGCT2760.10887573964497041No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACTCTCTCAAGTCGGCT2750.10848126232741617No Hit
CTGGGCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT2640.10414201183431952No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT2630.10374753451676529No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2580.10177514792899407No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2570.10138067061143984No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGATCTTCCTCTCAGAACCCCT2560.10098619329388561No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGCCCTCTCAGGCCGGCT2560.10098619329388561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT85450.0294.654753
GACCGTG80150.0294.631934
TTGGACC49250.0294.101531
ACCGTGT80400.0294.08275
GTGTCTC192700.0294.081458
CGTATCT46450.0294.047337
CCGTGTC192650.0293.92816
CGTGTCT192600.0293.851237
TGGACCG85650.0293.794532
CTGGGCC106900.0293.758181
GCCGTGT88200.0293.49495
TGTCTCA193200.0293.473089
GGCCGTG88050.0293.32484
GGCCGTA43050.0292.944244
GCCGTAT43050.0292.601625
CCGTATC46900.0292.16956
TTGGTCC23950.0291.920651
GTATCTC46800.0291.84838
CTGGACC36800.0290.190221
TTGGGCC24700.0289.028351
Produced by FastQC (version 0.11.5)