FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181599
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA147148.102467524600906No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG130977.212044119185679No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA94715.215337088860621No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT67253.703214224747934No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT66913.684491654689728No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT66793.6778836887868325No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG54713.012681787895308No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA54112.979641958380828No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGAG38392.11399842510146No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC32691.8001200447139025No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAGGAAAA32681.7995693808886615No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG31841.7533136195683898No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA29051.599678412326059No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA26111.4377832477051085No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT25101.3821662013557343No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG23251.2802933936860887No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA22851.2582668406764355No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA22421.2345882961910584No Hit
GATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGTAGCA21021.1574953606572724No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGA20861.1486847394534112No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC17500.9636616941723247No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGA16980.9350271752597757No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGA16680.9185072605025358No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT16070.8849167671628146No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG15400.8480222908716457No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA15320.8436169802697152No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA15150.8342556952406125No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT15110.8320530399396472No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAAG15050.8287490569881992No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT14560.8017665295513742No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAA13340.7345855428719321No Hit
GATAAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTATGTT13020.7169643004642097No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT11570.6371180458042169No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT11340.6244527778236664No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG10850.5974702503868413No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGAAGAAGATT9230.5082627106977462No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC8920.4911921321152649No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACACT7430.40914322215430704No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG7350.4047379115523764No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGACGATGCC7230.39812994564948045No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG7030.3871166691446539No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGCATAGA6910.38050870324175795No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT6720.3700460905621727No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT6520.3590328140573461No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA6430.35407683963017417No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTTAAGGCG6250.3441648907758303No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG6120.337006261047693No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGGAGAAGCCT5720.3149797080380399No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGCGGGGCAGCATAA5570.3067197506594199No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA5560.30616908683417865No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCGGGA5150.2835918699992841No Hit
AATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA4800.2643186361158376No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA4770.2626666446401137No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGGGATATTG4660.25660934256245904No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA4610.2538560234362524No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC4390.24174141928094317No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA4380.24119075545570184No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG4160.22907615130039263No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGT4100.22577216834894465No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA4060.22356951304797934No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCATGG4020.221366857747014No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA4000.22026553009653138No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA3840.2114549088926701No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGAACGGG3670.20209362386356752No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA3620.1993403047373609No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA3570.19658698561115426No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT3480.1916310111839823No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGCGA3260.17951640702867305No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTT3200.1762124240772251No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT3090.17015512199957047No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT3070.16905379434908782No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT2790.15363520724233062No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG2780.15308454341708932No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT2660.14647657751419335No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC2660.14647657751419335No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT2630.14482458603846937No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG2630.14482458603846937No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGG2520.13876728396081478No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT2450.13491263718412547No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA2430.1338113095336428No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGA2340.12885533510647085No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGAG2330.12830467128122952No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA2270.12500068832978153No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2200.12114604155309225No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATATTT2180.12004471390260958No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACAAGG2160.11894338625212694No Hit
GATTAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGGAAAGTA2160.11894338625212694No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGA2030.11178475652398967No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGG1990.10958210122302436No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGAAG1980.10903143739778302No Hit
GACGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAACGGACCTAGCA1960.10793010974730038No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTAAGACG1920.10572745444633505No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG1890.10407546297061107No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCATTT1820.10022081619392177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGAA108.4591674E-4295.0295
CGCTAGC81150.0294.818247
ACGCTAG81100.0294.818156
CTAGCTA45750.0294.67769
TGAACGC127800.0294.538333
CTGGCGG100300.0293.970589
CGCTGGC100650.0293.534527
AACGCTG100700.0293.38885
GCTAGCG35150.0293.321478
GCTAGCT46350.0293.09068
ACGCTGG101100.0292.2286
CTAGCGA21900.0291.632429
AACGCTA82000.0291.582345
ATTGAAC14550.0290.945041
GAACGCT176200.0290.898134
ATGAACG113750.0290.331852
ACGAACG47200.0288.752
CGAACGC47300.0288.139533
GATGAAC114100.0287.114381
AAACGCT5150.0286.407754
Produced by FastQC (version 0.11.5)