FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181599
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT5176428.504562249792126No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT150858.30676380376544No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT139987.708192225728115No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT130207.169643004642095No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT83774.612910864046609No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT56113.0897747234290938No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT39732.1877873776837977No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT36952.0347028342667084No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT36632.017081591858986No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCACCCTCTCAGACCAGCT35721.966971183762025No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT33271.8320585465778996No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT30621.6861326328889477No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT28551.5721452210639926No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT28301.5583786254329595No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGTTTGGCCTCTCAGCTCAGCT21481.1828258966183736No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT18671.0280893617255602No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT13670.752757449104896No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT11570.6371180458042169No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT10390.5721397144257403No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT9850.5424038678627086No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT8550.4708175705813358No Hit
CTGGACCGTCTCTCAGTTCCAATGTGACCGGTCAACCTCTCAGTCCGGCT7090.3904206520961019No Hit
TTGGACCGTTTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGATCGGCT6710.36949542673693136No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT6120.337006261047693No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT6110.33645559722245166No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT6030.33205028662052105No Hit
CTGGTCCGTATCTCAGTACCAGTGTGGGGGGTAGTCCTCTCAGACCCCCT6030.33205028662052105No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT5820.32048634629045314No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGCCCTCTCAGGTCGGCT5820.32048634629045314No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCTGGTCGGTCTCTCAACCCAGCT5500.30286510388273064No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT5390.29680780180507604No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT5260.28964917207693874No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT5030.27698390409638823No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT5020.27643324027114685No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT4820.26541996376632027No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT4770.2626666446401137No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT4700.2588119978634244No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT4700.2588119978634244No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCGTCCTCTCAGACCAGCT4500.2477987213585978No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3870.21310690036839408No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT3430.18887769205777563No Hit
CTGGACCGTGTTTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT3330.18337105380536237No Hit
TTGGACCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT3240.1784150793781904No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3220.17731375172770775No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT3070.16905379434908782No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2870.15804051784426124No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGGTCTCTCAACCCGGCT2540.1398686116112974No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2500.1376659563103321No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT2480.13656462865984945No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2470.13601396483460812No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCACCCTCTCAGGTCGGCT2420.1332606457084015No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGGTTAACCTCTCAGTCCCCCT2360.1299566627569535No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2360.1299566627569535No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT2330.12830467128122952No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGATCTTCCTCTCAGAACCCCT2270.12500068832978153No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT2150.1183927224268856No Hit
CTGGGCCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT2050.11288608417447232No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCACCCTCTCAGACCAGCT2020.11123409269874834No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGCCTTCCTCTCAGAACCCCT1970.1084807735725417No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCGGCCTCTCAGCCCGGCT1960.10793010974730038No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT1940.10682878209681772No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1910.10517679062109374No Hit
TTGGTCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT1850.10187280766964575No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT1820.10022081619392177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCTG108.4591674E-4295.0295
GGACCGT89450.0294.67023
CGTGTCT137000.0294.3547
GTGTCTC136950.0294.35388
ACCGTGT83300.0294.291725
TGGACCG89600.0294.17692
GTATCTC40450.0293.906048
CTGGGCC61950.0293.809541
GACCGTG83450.0293.762734
CCGTGTC137350.0293.60396
CTGGACC29500.0293.51
CGTATCT40400.0293.17457
GGCCGTA36650.0292.987734
TTGGACC60500.0292.80581
TGTCTCA137800.0292.53819
GCCGTGT34150.0292.40855
GGCCGTG34400.0290.283424
CCGTATC41350.0287.509066
TTGGTCC11250.0287.133331
GCCGTAT37500.0286.346655
Produced by FastQC (version 0.11.5)