FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207004
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG204989.90222411161137No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT130086.283936542289038No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA72303.492686131668953No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTAG58012.8023613070278834No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA47212.2806322583138487No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC46252.2342563428726017No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT45752.210102220246952No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA38941.881123070085602No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG37971.8342640721918417No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA36521.7642171165774574No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT33641.6250893702537148No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT31891.5405499410639407No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA29421.421228575293231No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGCGCCCC29181.409634596432919No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG26701.2898301482096963No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGTGGCCC26481.2792023342544105No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT25991.2555312940812737No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT21931.0593998183609978No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG21221.0251009642325752No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAA20690.9994975942493866No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACATT20040.9680972348360418No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT19960.9642325752159379No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT19230.9289675561824893No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC18400.8888717126239107No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA18220.8801762284786768No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT17850.862302177735696No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA17000.8212401692720913No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG16280.7864582326911557No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG15380.7429808119649861No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAACACCCC13650.6594075476802381No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATTT12910.6236594461942764No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA12120.5854959324457498No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTTGA11210.5415354292670673No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC10840.5236613785240865No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGGTTT9940.48018395779791695No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA9910.47873471044037796No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT9800.47342080346273496No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGATCATG9740.4705223087476571No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC9740.4705223087476571No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATTATT9690.46810689648509207No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACATA9510.4594114123398582No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT9170.4429866089544163No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA8820.42607872311646156No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGAT8770.4236633108538965No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG8550.4130354968986107No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG8440.4077215899209677No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT8430.4072385074684547No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGCTAGGA8400.40578926011091576No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATTACT7970.385016714652857No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG7940.38356746729531793No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA7760.3748719831500841No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCACTGATTT7580.36617649900485016No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACATC7270.3512009429769473No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGCGGCCC7100.3429885412842264No Hit
GATGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGAAGCACCTT6610.31931750111108964No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT6600.31883441865857665No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT6450.3115881818708817No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTT6380.30820660470329075No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG5970.28840022415025796No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG5950.28743405924523197No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA5900.285018646982667No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGCGGCCC5880.284052482077641No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAAG5770.2787385750999981No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCGGGA5560.2685938435972252No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAT5550.2681107611447122No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC5320.25699986473691333No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC5290.25555061737937435No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTCTTTA5210.25168595775927033No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACAGAACT5170.24975362794921835No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC4980.24057506135147147No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA4920.23767656663639347No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACACGG4680.2260825877760816No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGTCC4460.21545477382079573No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAGGTGGCCC4190.20241154760294489No Hit
GATGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT4140.1999961353403799No Hit
GACGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAACGGACCTAGCA4070.19661455817278892No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAG4060.19613147572027592No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGG3930.189851403837607No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC3920.18936832138509402No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCC3750.1811559196923731No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT3730.1801897547873471No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAGCGGAGATTGAG3720.1797066723348341No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTG3710.1792235898823211No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA3620.17487584780970417No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGGGCAGTA3520.17004502328457421No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGG3510.16956194083206122No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAAGGGCCC3510.16956194083206122No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGGGGCCC3390.16376495140190528No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAGGTAGCAA3360.1623157040443663No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTCTCTTC3350.1618326215918533No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT3180.15362021989913238No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC3180.15362021989913238No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT3090.14927247782651543No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTAG3020.14589090065892446No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT3000.14492473575389847No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA2880.13912774632374253No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA2850.13767849896620354No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT2810.13574616915615156No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCGTCTT2770.1338138393460996No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGTAA2730.1318815095360476No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG2710.1309153446310216No Hit
GATGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAGCGGAGTTTTGT2690.12994917972599562No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA2690.12994917972599562No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA2660.12849993236845667No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCTCT2640.12753376746343068No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTT2620.12656760255840466No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACCACTTC2580.12463527274835268No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACATGT2570.12415219029583968No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGACGATGCC2470.11932136577070973No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA2430.11738903596065778No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT2380.11497362369809279No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT2360.1140074587930668No Hit
GACGAACGCTGGCGGCGTGCTTCATACATGCAAGTCGAACGCGAATCGGT2340.1130412938880408No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA2320.11207512898301483No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT2250.10869355181542385No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAAGACG2110.10193039748024192No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA2100.10144731502772894No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG2090.10096423257521595No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCATTTT2090.10096423257521595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGC64800.0295.07
TGTACCT108.4600237E-4294.99997295
ACGCTAG64850.0294.772556
TGAACGC117900.0294.62473
CTGGCGG141300.0294.37379
CTAGCTA42400.0294.304269
AACGCTG142050.0293.961645
CGCTGGC141800.0293.95987
AAACGCT19900.0293.517584
ACGCTGG142400.0293.031956
GCTAGCT42900.0292.937078
ATAAACG19800.0292.765142
TAAACGC19900.0291.293983
GCTAGCG22400.0291.04918
GATAAAC20050.0289.114721
ATGAACG119050.0287.442232
ACGAACG66400.0286.780882
GATGAAC119600.0285.997071
GACGAAC66900.0284.417051
GAACGCT189100.0284.391854
Produced by FastQC (version 0.11.5)