FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207004
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3917518.924755077196576No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2224910.748101485961623No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT167868.109022047883133No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT120415.816795810708972No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT83534.0351877258410465No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT75653.654518753260807No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT53982.6076790786651465No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT50012.415895345017488No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTTCGGCT40641.9632470870128114No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT39221.8946493787559662No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT38951.8816061525381154No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGTTCAACCTCTCAGTCCGGCT31321.5130142412707002No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT30461.4714691503545825No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT26211.2661591080365595No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT24471.1821027612992985No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT23971.1579486386736488No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT21691.047805839500686No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT21571.04200885007053No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT14290.6903248246410697No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT14030.6777646808757319No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT14000.6763154335181929No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTCCGGCT13950.6739000212556279No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT12670.6120654673339646No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT11490.5550617379374312No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT10800.5217290487140345No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT10570.5106181523062356No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT10530.5086858224961837No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTTCGGCT9960.48115012270294294No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT9890.477768545535352No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT9510.4594114123398582No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT8180.3951614461556298No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGTCCACCCTCTCAGGTCGGCT7950.3840505497478309No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCGGTCTCTCAACCCGGCT7800.37680431296013606No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT7690.37149040598249305No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCAGCT7410.3579640973121292No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATTAACCTCTCAGTTCGGCT7350.3550656025970513No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGTCGGCT7130.3444377886417654No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT6690.3231821607311936No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGCCGGCT6540.3159359239434987No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT6330.3057911924407258No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT6160.29757879074800486No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT5850.282603234720102No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACTCTCTCAAGTCGGCT5830.281637069815076No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT5450.2632799366195822No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT5200.25120287530675733No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT4860.23477807192131556No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT4710.2275318351336206No Hit
CTGGGCCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT4280.2067592896755618No Hit
ATGGGCCGTGTCTCAGTCCCATTGTGGCTGATCATCCTCTCAAACCAGCT4230.20434387741299684No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTGACCTCTCAGTCCCCCT4220.20386079496048384No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT4160.2009623002454059No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGTCCGATCAGCCTCTCAGCTCGGAT4070.19661455817278892No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT4060.19613147572027592No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3850.18598674421750305No Hit
TTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3770.1821220845973991No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT3760.1816390021448861No Hit
CTGGACCGTTTCTCAGTTCCAATGTGGCCGGTCATCCTCTCAGATCGGCT3480.16811269347452223No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT3340.1613495391393403No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGACCTCTCAGTCCGGCT3260.15748487951923634No Hit
TTGGACCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT3250.15700179706672335No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGTTCACCCTCTCAGGTCGGCT3080.14878939537400243No Hit
TTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2960.1429924059438465No Hit
CTGGTCCGTATCTCAGTACCAGTGTGGGGGATAATCCTCTCAGACCCCCT2720.1313984270835346No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2600.12560143765337867No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT2600.12560143765337867No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCATCCTCTCAGACCGGCT2510.12125369558076173No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2500.12077061312824873No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCCCGGCT2480.11980444822322274No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT2430.11738903596065778No Hit
CTGGACCGTCTCTCAGTTCCAATGTGACCGGTCAACCTCTCAGTCCGGCT2400.11593978860311878No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT2320.11207512898301483No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT2220.10724430445788488No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGATCTTCCTCTCAGAACCCCT2200.10627813955285889No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT2150.1038627272902939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT74100.0295.03
CGTGTCT165400.0294.732487
GTGTCTC165450.0294.554238
TGTCTCA165350.0294.5549
GCCGTGT79950.0294.262025
CCGTGTC165750.0294.021126
GACCGTG69650.0293.941164
CGTATCT37250.0293.812077
TGGACCG74600.0293.22052
ACCGTGT70000.0292.471475
GTATCTC37400.0292.23938
GGCCGTG80600.0292.071964
TTGGACC50600.0291.501981
CTGGACC24450.0291.380341
GGCCGTA35950.0290.89714
TGGTCCG16900.0290.636082
CTGGGCC84950.0290.13831
CCGTATC37900.0288.773076
CTGGTCC7050.0288.723421
GCCGTAT36300.0288.498635
Produced by FastQC (version 0.11.5)