FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202091
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGGCC86884.299053396737113No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG85734.242148339114557No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG84674.189696720784202No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA80553.985828166519043No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA79683.9427782533611095No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTTTT74413.682004641473396No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG63323.1332419553567448No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT62773.1060264930155226No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT62483.0916765219628783No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA59772.9575785166088546No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGAACTTA54722.7076910896576294No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT52102.578046523595806No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGA50752.511244934212805No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA49792.463741581762671No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGTGG45362.2445334032688247No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG42532.104497478858534No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA34661.715068954085041No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT26291.3008990999104364No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG25441.258838839928547No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT23361.1559149096199237No Hit
GATGAACGCTAGCTGCAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA18120.8966257774962764No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC17960.8887085520879208No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT16820.8322983210533869No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC15930.7882587547194085No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA15100.7471881479135637No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA15080.7461984947375193No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG12800.6333780326684514No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG11990.5932970790386509No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG11910.5893384663344731No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG11670.5774626282219396No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA10680.5284747960077391No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA10660.5274851428316947No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC10180.5037334666066277No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA9840.48690936261387197No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAAG9670.47849731061749406No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC9670.47849731061749406No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG9200.45524046098044935No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA8840.4374267038116492No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT7990.3953664438297599No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC7530.37260442078073736No Hit
GATGAACGCTGGCGGCGCATTTAAGACATGCAAGTCGAACGAAAATACTC7400.36617167513644844No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGAAGAAGATC7290.3607285826682039No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA7110.35182170408380387No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGA6890.34093551914731485No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGAC6880.3404406925592926No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGAAGAT6770.3349976000910481No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC6620.32757520127071466No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG6530.3231217619785146No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT6340.31372005680609233No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGAAGAAGATT6270.3102562706899367No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA6090.3013493921055366No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG5780.28600976787684756No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA5380.26621670435595846No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGA5180.25632017259551393No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG4720.23355814954649146No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGGTCAAGG4660.23058919001835804No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAAAGG4640.2295995368423136No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT4360.21574439237769125No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAAGTTTCAT4290.21228060626153566No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT4260.21079612649746896No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT4120.2038685542651578No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG4050.20040476814900215No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG3900.19298236932866877No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT3810.18852893003646873No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG3760.18605479709635758No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTAG3690.182591010980202No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT3650.18061170462811307No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA3600.17813757168800193No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT3580.1771479185119575No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG3250.160818641107224No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA3070.15191176252282387No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA3020.14943762958271273No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGAGA2960.14646867005457936No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACACGG2940.14547901687853493No Hit
ATTGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGTACGTGAAAGGGT2940.14547901687853493No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG2860.1415204041743571No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGGCC2800.1385514446462237No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA2760.1365721382941348No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGATTT2750.1360773117061126No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAAGAGG2710.13409800535402366No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG2700.13360317876600145No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC2680.132613525589957No Hit
AGTGAACGCTGGCGGAACGCTTGACACATGCAAGTCGAACGGTAACGTGG2680.132613525589957No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC2670.13211869900193476No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA2670.13211869900193476No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAATTACGA2620.12964456606182365No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCGGGG2610.12914973947380143No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAATTCTT2560.12667560653369028No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGA2540.12568595335764582No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCATGG2520.12469630018160135No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT2350.11628424818522348No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAATGAC2340.11578942159720126No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAA2250.11133598230500122No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG2240.11084115571697899No Hit
GATGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGAAGCACCTT2210.1093566759529123No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTGAGA2100.1039135834846678No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT2030.10044979736851221No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGTC2030.10044979736851221No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAC108.4598735E-4295.0295
CTGGCGG135950.0294.5669
AACGCTG136350.0294.45915
ACGCTAG65900.0294.328556
TGAACGC109050.0294.32373
CTAGCTA33250.0294.11289
CGCTAGC65950.0294.105387
CGCTGGC136500.0293.91947
ACGCTGG136850.0293.27556
GCTAGCG30850.0289.262578
GCTAGCT36250.0288.896558
AACGCTA67150.0288.62995
TAAACGC4450.0288.37083
CGAACGC90900.0286.88673
AGATACG4950.0286.0606295
ACGAACG91250.0285.4632
AAACGCT4500.0285.16674
ATAAACG4500.0285.16672
GACGAAC91150.0284.805271
GAACGCT206500.0281.71434
Produced by FastQC (version 0.11.5)