FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145580
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT89646.157439208682511No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC77505.3235334523973075No Hit
GATTAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT46113.167330677290837No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG42552.922791592251683No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGCGACCC39852.7373265558455833No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG38992.6782525072125294No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT34732.3856298942162386No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA34532.371891743371342No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA27371.8800659431240554No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT26301.8065668361038605No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGTTA25781.7708476439071301No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC23041.5826349773320512No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT20881.4342629482071714No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG20851.432202225580437No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT19381.331226816870449No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT16321.1210331089435361No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA16051.1024866053029263No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG15781.0839401016623162No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGATGCAT14851.0200577002335485No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGAAAGTT14751.0131886248111004No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGTCC14741.0125017172688555No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA14470.9939552136282457No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTT14040.9644181893117186No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGACCATA12330.8469569995878554No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC11990.8236021431515318No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT11980.822915235609287No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG11410.7837615057013325No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC10960.752850666300316No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATC10740.7377387003709301No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT10120.6951504327517516No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTG9930.6820991894491002No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA9340.6415716444566562No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC9300.6388240142876769No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTTAA8980.6168429729358428No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAT8220.564637999725237No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA7830.5378486055776893No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA7600.5220497321060585No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA7550.5186151943948345No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT7440.5110592114301415No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGCTTATTT7330.5035032284654486No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG7290.5007555982964693No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTGCC7090.48701744745157305No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA7060.48495672482483865No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCAGCA6670.4581673306772908No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT6550.4499244401703531No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGCTGATT6400.4396208270366808No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGGGACCC6400.4396208270366808No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATGA6310.43343865915647756No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG5730.39359802170627833No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACTTGT5700.39153729907954393No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGAGGCGG5630.3867289462838302No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC5170.35513119934056875No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA5100.35032284654485507No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG5000.34345377112240694No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGTGACCC4950.3400192334111829No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTG4920.33795851078444844No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA4870.33452397307322435No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATTACA4820.33108943536200025No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC4800.32971562027751067No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA4480.30773457892567657No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA4170.2864404451160874No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT4020.2761368319824152No Hit
GATGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT3990.2740761093556807No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC3860.2651463113064982No Hit
GATAAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTATGTT3820.2623986811375189No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGAAATACT3690.2534688830883363No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGAGATAAAG3600.24728671520813297No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAAAGCAC3520.24179145487017448No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG3510.24110454732792966No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT3510.24110454732792966No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC3490.23973073224344005No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA3460.2376700096167056No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT3380.2321747492787471No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG3290.22599258139854375No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGAACAGG3100.2129413380958923No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT3100.2129413380958923No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT3070.21088061546915784No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT3040.20881989284242342No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGGGACCC3000.20607226267344414No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA3000.20607226267344414No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATGCTT2950.20263772496222007No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACAAGG2930.20126390987773046No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC2770.19027338920181341No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC2710.18615194394834456No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGGGAGAATTG2700.18546503640609974No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT2620.1799697760681412No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTTTACT2510.1724137931034483No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTGAAGA2460.16897925539222422No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCATGG2420.16623162522324497No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATGTTT2310.158675642258552No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGTTT2220.15249347437834868No Hit
GATAAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTTTTC2220.15249347437834868No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT2210.15180656683610386No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTG2160.1483720291248798No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGAC2140.14699821404039018No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGGGATTGTTA2130.14631130649814533No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG2110.14493749141365572No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGGACGAAGCGACCC2100.1442505838714109No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT2070.14218986124467647No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAGTTAGA2050.14081604616018684No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC2050.14081604616018684No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATTT2030.13944223107569723No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG2010.1380684159912076No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAAAGTCGTT2010.1380684159912076No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA1870.12845171039978018No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGAAGCTGCGT1820.12501717268855614No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACATA1780.12226954251957686No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA1760.12089572743508724No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGTC1760.12089572743508724No Hit
GACGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG1720.11814809726610799No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGCGACCC1670.11471355955488391No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATACTT1670.11471355955488391No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC1660.1140266520126391No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACATGT1650.11333974447039429No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC1640.11265283692814947No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTTACA1630.11196592938590465No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC1590.1092182992169254No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGGG1580.1085313916746806No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATCTT1530.10509685396345651No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATACT1480.10166231625223245No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG1470.10097540870998764No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA1460.10028850116774281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAACG12900.0295.02
CTAGCTA8000.0295.09
CAGACAC157.2274033E-6295.0295
TCCAGAA206.166556E-8294.99997295
CGCTGGC122600.0294.75947
CTGGCGG122450.0294.75919
ACGCTGG122700.0294.519176
TGAACGC72150.0294.386723
GATAAAC12950.0293.861021
GCTGGCG122850.0293.799358
CGCTAGC22750.0293.703287
ACGCTAG22750.0293.703286
AACGCTG123200.0293.323855
AAACGCT13100.0292.74814
GCTAGCG14750.0292.08
TAAACGC13050.0291.60923
ATGAACG66800.0290.804662
TTAACGC10300.0290.70393
CTAGCGA9450.0290.317449
GAACGCT126050.0285.63864
Produced by FastQC (version 0.11.5)