FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11670271
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA343160.29404629935328835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG18850.046.3033943
TATGCCG21100.041.6926546
CGTATGC22100.039.65136344
CTCGTAT25550.033.89145342
GTATGCC30900.028.80541645
ATGCCGT39500.022.79451247
ACTTAGG42500.021.51072132
GCCGTCT42200.021.17205649
TCTCGTA41450.020.8910541
CACTTAG46000.019.64921631
TGCCGTC47450.019.2658948
TCACTTA48200.018.89583630
CCCCCCC165650.018.5787351
TAGGCAT50000.018.2142635
ATCTCGT47300.018.08869440
TTAGGCA53200.017.18439134
CTTAGGC53200.017.05425833
CCCCCCT81200.014.9501353
CCGTCTT61400.014.71983850
TCGACCG6800.013.69798431
Produced by FastQC (version 0.11.5)