FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10228842
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA523610.5118956769495511TruSeq Adapter, Index 16 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA273080.2669705915879823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG70400.060.22625445
TATGCCG70800.059.8804348
CGTATGC73500.057.73021746
CTCGTAT74450.055.46745344
GTATGCC80700.052.70651647
CGTCCCG86750.048.67308435
TCCCGAT86600.048.0401337
GTCCCGA88900.047.4971136
ATGCCGT89450.047.4334549
GCCGTCT89750.047.35222651
ACCCGTC90250.047.24855432
TGCCGTC92350.046.39125450
CCCGATC87750.046.31070738
TCTCGTA88500.046.0773243
CCGATCT91150.044.84863339
GATCTCG93800.043.6194341
ATCTCGT94350.043.0741242
CACCCGT99700.042.8741531
TCACCCG104000.040.97057730
CCGTCCC104900.040.8793934
Produced by FastQC (version 0.11.5)