FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11057769
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG12150.030.6652943
TATGCCG12900.029.4166246
CGTATGC15000.026.219344
CTCGTAT18400.019.68670742
GTATGCC22700.017.3253745
CGTATCG12050.016.6001853
CCGTATC12250.014.9214072
CCCCCCC134750.013.6242971
GTATCGA9450.013.50334
GTCGCTA8550.012.91158627
GCCGTAT11450.012.6582911
ATGCCGT32950.012.56336147
CCCCCCT59800.012.1689543
TCGCTAT9350.011.80679728
AATCTCG32000.011.75076939
TCGACCG9550.011.5596931
GGGGCAT94550.011.27791
GCGGTAT13250.011.1991191
ACGTTCG7400.011.18534856
CGCTATG9600.011.14007529
Produced by FastQC (version 0.11.5)