FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18948991
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA283900.1498232808279871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG23550.039.84626445
TATGCCG25350.037.42136848
CGTATGC28250.033.2156646
CGTATCT34800.027.6581539
CTCGTAT34650.026.88281444
TCCGTAT36100.026.66137737
TATCTCG38250.024.62253841
GTATGCC41350.023.10915647
CCGTATC44300.021.6488438
GTTCCGT50000.019.4569635
ATGCCGT56050.017.54009249
CCCCCCC242550.016.7010631
GCCGTCT59700.016.69865851
AGTTCCG63000.016.53804234
GTATCTC62300.015.89299640
TCTCGTA61950.015.64840343
TTCCGTA63700.015.16426536
TGCCGTC67600.015.15537450
CGACCGT23600.014.91124232
TCGACCG23200.014.87083631
Produced by FastQC (version 0.11.5)