FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11503397
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA235290.20453958078644074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG12450.041.28781543
TATGCCG14800.033.7979646
CGTATGC17250.029.99889444
CTCGTAT21750.023.1581742
TATCTCG22550.022.33562339
GTATGCC25450.020.6039945
ACGCCAA25450.019.51990932
CGCCAAT28750.018.23908833
CCCCCCC153050.016.841951
GTCACGC32550.015.89844629
ACGTTCG8350.015.6953126
AGTCACG33200.015.37915128
CCCCCCT75200.015.2262534
GCCGTCT35500.015.1582949
ATGCCGT35000.015.07982447
TCGACCG12000.014.94957931
CGACCGT12450.014.68620532
CCAATAT36900.013.83574635
TGCCGTC40000.013.36715248
CGCTATG8800.012.93736229
Produced by FastQC (version 0.11.5)