FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11205965
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG127180.11349312620555214TruSeq Adapter, Index 8 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24700.049.58208546
TCGTATG24400.049.3431743
CGTATGC27500.044.40867644
CTCGTAT31350.037.85421842
GTATGCC36750.033.32530645
AATCTCG41550.029.39149939
GCCGTCT43400.027.74020849
TCTCGTA45750.026.91923741
ATGCCGT47700.025.8191147
TGCCGTC51600.023.66667648
ATCTCGT52550.023.10784740
TTGAATC58100.021.7313536
CCGTCTT64250.018.63058550
GAATCTC71600.017.68237738
CGTATCG10150.016.9902763
ACACTTG79900.016.83955432
CCCCCCC144100.016.7644881
CTTGAAA77550.016.55573360
CCCGTAT14050.016.2115251
CGTCTTC73500.015.95667251
Produced by FastQC (version 0.11.5)