FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9779265
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG8150.033.86456743
TATGCCG9900.027.18061346
CGTATGC11250.024.53324744
CTCGTAT14400.018.20808442
ACGTTCG9200.016.1194216
GTCCGAT10900.015.8294381
CGATTGG8850.014.8085844
GTATGCC19000.014.5258245
CCGATTG11100.014.2925683
GCGATCG10300.014.06748141
CCCCCCC108200.014.0010061
CGTGCGA11300.013.1278738
CGTTCGA11950.012.9868717
GTCCGTT12100.012.5484281
CGATCGG13100.012.37769342
TCCGATT17250.012.196092
ATCGCGT3903.6652636E-711.49607913
GTTACGG11200.011.3931457
CCTACCG3652.0553052E-611.34190433
TTACGGA12550.011.2667868
Produced by FastQC (version 0.11.5)