FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11761137
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG10450.031.03314243
TATGCCG12350.026.81614146
CGTATGC14000.023.65747644
ACGTTCG11650.019.8346526
CTCGTAT17250.017.39972942
GTATCGA9600.016.885124
CGTGCGA13400.016.7339638
CGTATCG14100.016.1436713
GTATGCC21550.015.84900945
CGTTCGA14500.015.6981317
GCGATCG14550.015.41241
CCGTATC16800.014.5756052
CCCCCCC150700.014.3573931
GTCCGAT11750.013.8032481
CCCGTAT17600.013.724821
GTCCGTT13900.013.6542921
CGACCGT13650.013.1428232
CGATCGG17650.012.90064842
TCGACCG13950.012.86050731
ACTATCG8500.012.58633158
Produced by FastQC (version 0.11.5)