FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11162299
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA324160.290406125118132No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA186140.1667577619986707TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG30750.052.28193345
TATGCCG32500.050.20643648
CGTATGC35150.046.226946
CTCGTAT38900.041.86076444
GTATGCC41950.039.06134447
CGTTTCG49400.036.2441233
ATGCCGT49550.032.79058549
AATCTCG50950.032.6362441
TTCGGAA54400.032.53178436
CGGAATC54250.032.04985438
TCGGAAT55300.031.81490137
TCTCGTA52150.031.62150243
TTTCGGA57600.030.90386435
GCCGTCT52100.029.92644351
TGCCGTC55200.029.62111950
GTTTCGG61300.029.15213634
ACGTTTC63600.028.58620832
GTCACGT64300.028.38243729
AGTCACG65250.027.65160428
ATCTCGT60100.027.55293742
Produced by FastQC (version 0.11.5)