FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47163030
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATGC3704800.7855305310112602TruSeq Adapter, Index 8 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT401150.042.5986836
TCGCGCA439300.042.50961735
TCGTATG445950.042.2185644
TCTCGCG448900.041.79158433
CTCGCGC451050.041.5524834
CGTATGC453000.041.55158645
CTCGTAT459200.040.85787243
ACACGTC468050.040.55071312
ACGTCTG468650.040.47535714
ATCGGAA475850.040.096371
GCGCATC432050.039.53142537
TCTCGTA438150.039.2679842
CGTCTGA485300.039.07312415
TCGGAAG499550.038.855542
ATCTCGT441850.038.8428641
CACACGT489950.038.83444211
CTCTCGC488150.038.6472732
CACGTCT493300.038.46140713
CGGAAGA502900.038.4303973
GCACACG511100.037.23661810
Produced by FastQC (version 0.11.5)