FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52485366
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGC6345241.2089541301855455TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC722000.043.2849845
TCGTATG730450.042.69493544
ACCGCTC735950.042.17788331
CTCGTAT742900.041.92491543
ACACGTC755600.041.8816112
TCACCGC751650.041.7519129
ACGTCTG759900.041.57753814
TATCTCG725750.041.5303540
ATCGGAA766600.041.39441
GTCACCG762250.041.1535928
TCTCGTA731700.041.0477342
CGTCTGA770050.040.97400315
CACACGT774300.040.90790611
ATCTCGT736450.040.76128441
CACCGCT765350.040.6017630
CACGTCT782650.040.42933313
GCACACG793750.039.9168410
CGGAAGA800600.039.840983
CGCTCAT797550.038.92585433
TCGGAAG829600.038.874142
Produced by FastQC (version 0.11.5)