FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52485366
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTCG6523551.2429274095183027Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT722550.043.4669410
AGCGTCG735900.042.7619
CGTGTAG732850.042.72030314
TCGTGTA741650.042.24736413
GTCGTGT742100.042.2460112
CGTCGTG750350.041.9435211
ATCGGAA758300.041.7660981
TAGTGTA749550.041.66723638
GATTAGT747350.041.62928835
GTAGATC751500.041.4893242
AGAGCGT761600.041.4502377
GATCTCG749950.041.37320345
GAGCGTC765300.041.2188538
GTGTAGG762600.041.11290715
GTAGGGA763600.041.09150717
AGATTAG765150.040.6902534
TGTAGGG774050.040.55012516
TAGATCT771550.040.37013243
ATTAGTG773250.040.258736
TCGGAAG791400.040.082732
Produced by FastQC (version 0.11.5)