FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50419101
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTCG2094220.4153624238559906Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT232200.041.87512610
AGCGTCG245100.039.7905779
GTCGTGT253550.038.5509412
TCGTGTA254150.038.4336213
ATCGGAA261050.037.8636931
CCGTGTA256850.037.6808438
CGTCGTG263300.037.4226411
GAGCCGT260550.037.1744635
AGAGCGT263250.037.118577
GTAGATC266950.036.50331542
GAGCGTC269750.036.1543358
GTGTAGG273650.035.9009915
GATCTCG274250.035.56247745
GTAGGGA277750.035.40556317
TGTAGGG282100.034.75332316
AAGAGCG285550.034.4880876
AGCCGTG282100.034.4216536
GCCGTGT283400.034.2017937
TCGGAAG295650.033.6353152
TAGATCT289750.033.63022643
Produced by FastQC (version 0.11.5)