FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53838351
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGC1968380.36560926615304395TruSeq Adapter, Index 13 (97% over 37bp)
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATGT668300.12413084494359791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG236100.040.9121944
CGTATGC249000.038.86417445
ACACGTC257600.038.01257712
CTCGTAT257050.037.514143
ACGTCTG260900.037.462814
AATCTCG248200.036.96539340
ATCTCGT261600.035.09239641
CGTCTGA278250.035.04615
TCTCGTA264000.034.7638242
TCGGAAG296300.034.235032
CACGTCT288050.033.9708113
ATCGGAA292750.033.7863961
CACACGT290800.033.70389611
CGGAAGA302850.033.0402033
GCACACG302850.032.41485610
CGTATCG107200.029.4840453
CCCGTAT120550.027.7183881
ATTCAGA353150.027.6844633
GTATCGA114200.027.5782974
GAGCACA365050.027.0651638
Produced by FastQC (version 0.11.5)