FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53838351
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTCG2053570.3814325591064258Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT232700.041.76722310
CGTGTAG246650.039.52204514
AGCGTCG257250.038.0179679
TAGTGTA256850.037.63044738
TCGTGTA259550.037.54429213
GTCGTGT259250.037.47528512
AGAGCGT261950.037.149677
ATCGGAA265550.036.990871
GTAGATC264300.036.647642
GAGCGTC268650.036.3730558
ATAGTGT269250.035.8887837
GTAGGGA276800.035.30943717
CGTCGTG278350.035.30411511
GAGTGTA277550.034.94849826
GATCTCG279250.034.6464645
TAGATCT282150.034.3312943
TGTAGGG285050.034.2876616
AAGAGCG292800.033.6307456
TAGGGAA300450.032.70203818
TCGGAAG304650.032.6259652
Produced by FastQC (version 0.11.5)