FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51161949
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGC3391950.6629829524281806TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC405750.041.38196645
TCGTATG413400.040.7529744
ACACGTC434900.039.17364512
CTCGTAT429350.039.06614743
ACGTCTG436000.038.987114
TATCTCG402000.038.82705340
ATCGGAA447900.038.2836881
TCGGAAG460700.037.646532
TCTCGTA418400.037.33064742
CGTCTGA455650.037.3063115
ATCTCGT420850.037.18953741
GAAGCTA454350.037.06725335
CGGAAGA468450.036.877623
CACGTCT462600.036.84255613
CACACGT463800.036.82480211
GCACACG471200.036.28945510
GCTATCT432400.036.1335138
AGCTATC436650.035.82274637
CTATCTC446450.035.08726539
ACCTGAA506600.033.4017131
Produced by FastQC (version 0.11.5)