FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53139226
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGC5397541.0157355321660124TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC632850.042.57555445
TCGTATG641700.042.0590344
CTCGTAT653750.041.1530543
TATCTCG614850.040.7178540
ACACGTC671200.040.68353312
ACGTCTG676050.040.35838714
ATCGGAA685800.039.880931
TCTCGTA630050.039.83790642
CGTCTGA686950.039.6719815
AGCTATC634050.039.4844837
GAAGCTA686200.039.2244935
ATCTCGT642050.039.06994641
CACACGT700000.039.06425511
CACGTCT703100.038.87289413
GCTATCT646300.038.7745238
CGGAAGA715300.038.5314673
TCGGAAG726200.038.1822132
CTATCTC665450.037.69270339
GCACACG728700.037.58748210
ACCTGAA739850.036.61542531
Produced by FastQC (version 0.11.5)